Overview

  • Product nameAnti-Collagen I antibody [5D8-G9]
    See all Collagen I primary antibodies
  • Description
    Mouse monoclonal [5D8-G9] to Collagen I
  • SpecificityAb23446 is highly specific for type I collagen. The native triple-helical conformation is required for reaction. It has no cross reactivity with type II, III, V and VI collagens. There is no evidence for cross reactivity with other connnective tissue proteins (laminin, fibronectin, elastin).
  • Tested applicationsELISA, ICC/IF, IP, Flow Cyt, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Sheep, Goat, Cow, Dog, Human, Pig

    Does not react with

    Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cat, Kangaroo
  • Immunogen

    Full length native protein (purified) corresponding to Human Collagen I.
    Database link: P02452

  • EpitopeThe epitope recognised by ab23446 is located near the C terminal of the molecule.
  • Positive control
    • human and bovine skin

Properties

Applications

Our Abpromise guarantee covers the use of ab23446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
ICC/IF Use at an assay dependent concentration. PubMed: 20161070
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 20161070
WB Use at an assay dependent concentration. Predicted molecular weight: 134 kDa. Conditions need to be native to detect Collagen I with this monoclonal antibody.
IHC-Fr Use at an assay dependent concentration. Note: If fixation of tissue is required, acetone or ethanol is recommended.

Target

  • FunctionType I collagen is a member of group I collagen (fibrillar forming collagen).
  • Tissue specificityForms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • Involvement in diseaseDefects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
    Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
    Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
    Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
    Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • Sequence similaritiesBelongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications
    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha 1 type I collagen antibody
    • Alpha 2 type I collagen antibody
    • Alpha-1 type I collagen antibody
    • CO1A1_HUMAN antibody
    • COL1A1 antibody
    • COL1A2 antibody
    • Collagen alpha-1(I) chain antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen I alpha 2 polypeptide antibody
    • Collagen Of Skin Tendon And Bone antibody
    • Collagen Type 1 antibody
    • Collagen type I alpha 1 antibody
    • Collagen type I alpha 2 antibody
    • OI4 antibody
    • Osteogenesis Imperfecta Type IV antibody
    • Pro alpha 1(I) collagen antibody
    • Type I procollagen antibody
    see all

Anti-Collagen I antibody [5D8-G9] images

  • Immunohistochemistry (Frozen sections) analysis of sheep vagina tissue labelling Collagen I with ab23446.

  • Immunohistochemistry (Frozen sections) analysis of human endometrium tissue labelling Collagen I with ab23446.

References for Anti-Collagen I antibody [5D8-G9] (ab23446)

This product has been referenced in:
  • Jurgens WJ  et al. One-step surgical procedure for the treatment of osteochondral defects with adipose-derived stem cells in a caprine knee defect: a pilot study. Biores Open Access 2:315-25 (2013). IHC ; Goat . Read more (PubMed: 23914338) »
  • Flagler DJ  et al. Intracellular Flow Cytometric Measurement of Extracellular Matrix Components in Porcine Intervertebral Disc Cells. Cell Mol Bioeng 2:264-273 (2009). Flow Cyt, ICC/IF ; Pig . Read more (PubMed: 20161070) »

See all 5 Publications for this product

Product Wall

Thank you for contacting Abcam

I found one anti-Collagen I antibody that has been tested in Flow cytometry and would be backed by our AbPromise guarantee to work against human sample in flow cytometry for 6 months after purchase. However, this...

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DISCOUNT CODE:

100%ABR-T71OF für ab23446
100%ABR-T71OS für ab82354

Ablaufdatum: 31. August 2012

Es freut uns zu hören, dass Sie unser Angebot unsere Antikörper ab23446 und ab82354 inRatteund Schwei...

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Vielen Dank für Ihren Anruf.

Wie versprochen habe ich unsere Kollagen-Antikörper nach den von Ihnen genannten Kriteriengeprüft. Leider habe ich keinen perfekten Treffer gefunden, dafür aber vielleicht ein paar Kandidaten, ...

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Thank you for your enquiry.

I'm happy to help answer your questions:

1. I can recommend the following collagen I antibody, tested in WB and pig samples, and also this should be suitable for denaturing conditions. I can recommend t...

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Gracias por contactarnos.

Para la detección del colágeno I en borrego mediante WB, el anticuerpo ab23446 está testado y garantizado por nuestra garantía Abpromise:

http://www.abcam.com/Collagen-I-antibody...

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Thank you for contacting us.

We have a PE- conjugated antibody against cytokeratin, catalogue item ab52460:

Click here (or use the following: http://www.abcam.com/index.html?datasheet=52460).

For collagen I, we have onl...

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Thank you very much for your interest in ab23446 and ab90395. These two antibodies are not the same clone and come from different immunization experiments. ab90395 has not been tested for it's suitability in flow cytometry. I cannot rec...

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Abreviews
Application ELISA
Sample Human Purified protein (Human Collagen Type I)
Specification Human Collagen Type I
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Type Sandwich (Detection)
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Abcam user community

Verified customer

Submitted Jun 12 2008

Thank you for your enquiry. I have received the following protocol details from the source of this antibody: They recommend the cutting of fixed (using methanol or acetone) 6 um thick frozen sections, incubating with the antibody, washing twice...

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Thank you for your enquiry. The native triple-helical conformation is required for reaction. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"