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Full length native protein (purified) corresponding to Cow Collagen I.
Production of this antibody has been changed on 23rd June 2016. The following lots are from ascites and are still in stock as of 23rd June 2016 : GR210978, GR175242, GR158374. Lot numbers higher than GR210978 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Our Abpromise guarantee covers the use of ab6308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 3.5 - 7 µg/ml.
(amplification required).Use on unfixed tissue or acetone fixed tissue.
|Dot blot||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 17230415|
|Electron Microscopy||Use at an assay dependent concentration. PubMed: 17016762|
|Indirect ELISA||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Use under non reducing condition. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa).
The antibody is reactive with the native (non-denaturing, helical) form of collagen type I and not reactive when tested on thermally denatured molecules. Use native (non-denaturing) conditions.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 17016762
Fix in Zamboni's solution (2% paraformaldehyde, 0.2% picric acid in phosphate-buffered saline (PBS), pH 7.6) for 2 h at 4C, store in 20% sucrose in 0.5 mM PBS at 4C.
Immunoperoxidase staining of unfixed frozen tissue sections with ab6308. Picture of human kidney cortex showing two glomeruli and surrounding tubulointerstitium.
ab6308 at a 1/1000 dilution staining in rabbit polymer scaffold with stem cells by Immunocytochemistry/ Immunofluorecence, incubated for 2 hours at 20°C. PFA fixed. Permeabilized using 0.5% Triton X-100. Blocked with 3% BSA for 2 hours at 4°C. Secondary used at 1/1000 dilution monoclonal donkey anti-mouse IgG (H+L) conjugated to FITC.1st column: DAPI stain (blue)2nd column: collagen type I (green)3rd column: merge image
This image is courtesy of an anonymous Abreview