Overview

  • Product nameAnti-Collagen I [COL-1] antibodySee all Collagen I primary antibodies ...
  • Description
    Mouse monoclonal [COL-1] to Collagen I
  • Specificityab90395 will not cross react with collagen 2-11, or thermally denatured collagen.
  • Tested applicationsDot Blot, IHC-Glut, IHC-R, IP, Indirect ELISA, WB, ELISA, IHC-Fr, ICC/IF, Electron Microscopy, IHC-FoFr, IHC-P more details
  • Species reactivity
    Reacts with: Rat, Rabbit, Cow, Human, Pig, Deer
  • Immunogen

    Full length native protein (purified) corresponding to Cow Collagen I.
    Database link: P02452

  • Positive control
    • Connective tissue fibres. Pig skin tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab90395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Dot Blot Use at an assay dependent concentration.

Assay dependent

IHC-Glut 1/100 - 1/800. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-R Use at an assay dependent concentration.
IP 1/50.
Indirect ELISA Use at an assay dependent concentration.
WB 1/500 - 1/3000. Use under non reducing condition. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa). Use native (non-denaturing) conditions, as the antibody does not recognise denatured protein.
ELISA Use at an assay dependent concentration.

Assay dependent

IHC-Fr 1/2000.
ICC/IF 1/2000.
Electron Microscopy 1/100 - 1/1000. PubMed: 17016762for Gold labeling
IHC-FoFr 1/100. PubMed: 17016762Fix in Zamboni's solution (2% paraformaldehyde, 0.2% picric acid in phosphate-buffered saline (PBS), pH 7.6) for 2 h at 4C, store in 20% sucrose in 0.5 mM PBS at 4C.
IHC-P Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionType I collagen is a member of group I collagen (fibrillar forming collagen).
  • Tissue specificityForms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • Involvement in diseaseDefects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
    Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
    Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
    Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
    Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • Sequence similaritiesBelongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications
    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P02452 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Alpha 1 type I collagen antibody
    • Alpha 2 type I collagen antibody
    • Alpha-1 type I collagen antibody
    • CO1A1_HUMAN antibody
    • COL1A1 antibody
    • COL1A2 antibody
    • Collagen alpha-1(I) chain antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen I alpha 2 polypeptide antibody
    • Collagen type I alpha 1 antibody
    • Collagen type I alpha 2 antibody
    • Type I procollagen antibody
    see all

Anti-Collagen I [COL-1] antibody images

  • ab90395 staining Collagen I in porcine ligament by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in paraformaldehyde and an enzymatic antigen retrieval step was performed. Samples were then blocked using 1% BSA for 30 minutes at 20°C, then incubated with ab90395 at a 1/400 dilution for 1 hour at 20°C. The secondary used was an anti-mouse polyclonal conjugated to biotin-maleimide at a 1/200 dilution.

    See Abreview

  • ab90395 staining Collagen I in Pig skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/500 in PBS, Tween 0.01% + Donkey serum 1%) for 16 hours at 4°C. A Cy2® -conjugated Donkey anti-mouse IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Collagen I [COL-1] antibody (ab90395) at 1/1000 dilution

    Lane 1 : Recombinant Human Collagen I at 3 µg
    Lane 2 : Pig skin whole cell lysate extracted in Laemmli buffer at 20 µg

    Secondary
    HRP-conjugated Goat anti-mouse monoclonal IgG at 1/3000 dilution
    developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 130 kDa
    Observed band size : 130,140 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 250 kDa (possible dimer),400 kDa (possible multimer).

    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-Collagen I [COL-1] antibody (ab90395)

This product has been referenced in:
  • Zong ming W  et al. Bone formation in rabbit cancellous bone explant culture model is enhanced by mechanical load. Biomed Eng Online 12:35 (2013). WB ; Rabbit . Read more (PubMed: 23597232) »
  • Song Z  et al. Repair of abdominal wall defects in vitro and in vivo using VEGF sustained-release multi-walled carbon nanotubes (MWNT) composite scaffolds. PLoS One 8:e64358 (2013). IHC-P ; Rat . Read more (PubMed: 23717603) »

See all 5 Publications for this product

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these antibodies.

The details you have kindly provided will enable us to investigate t...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate buffer at pH=6.05
Sample Sheep Tissue sections (tendon)
Specification tendon
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 13 2013

Application Immunohistochemistry (Frozen sections)
Sample Pig Tissue sections (Ureter from Minipig)
Specification Ureter from Minipig
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Ms. Beate Thode

Verified customer

Submitted Feb 20 2013

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Ureter)
Specification Ureter
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Ms. Beate Thode

Verified customer

Submitted Feb 19 2013

Thank you very much for contacting us with your question.

We don't have any data specifically against pro-collagen (recombinant testing in WB, for example), but since mature collagen is created by cleavage of the pro-collagen, I would expect ...

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ab90395 is in form of ascites. The concentration is not determined.

Thank you for contacting us.

I have looked at the datasheet and indeed the information is confusing.

Reducing and non-reducing conditions refers to whether or not there are reagents present that disrupt disulfide bonds between cyst...

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Thank you very much for contacting us with your enquiry.

The AMELX protein ab139212 can be diluted in any buffer. However, all of our products are intended for research use only and are not recommended for in vitro studies. This protein conta...

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Vielen Dank für Ihre Anfrage.

Könnten Sie mir bitte noch mitteilen,mitwelchenCCR3 Polyclonal Antibody Sie den ab36827 vergleichen möchten, da wir eine sehr große Auswahl an polyklonalen Antikörpern haben?

Ich...

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Thank you for contacting us.

Unfortunatley we do not carry any polyclonal anti-Collagen I antibodys which will react with porcine that are not from a rabbit host. We do have two mouse monoclonal antibodies which we gaurantee in IHC-Fr on p...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"