Overview

  • Product name
    Anti-Collagen I + II + III + IV + V antibody
    See all Collagen I + II + III + IV + V primary antibodies
  • Description
    Rabbit polyclonal to Collagen I + II + III + IV + V
  • Specificity
    ab24117 recognises collagen I, II, III, IV and V. Antisera to specific collagen types were blended so to achieve equal reaction to any of the collagen types used.
  • Tested applications
    Suitable for: IHC-Fr, Dot blot, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein. Immunogens are extensively purified native collagen type I, II, III, IV and V.

  • Positive control
    • Human kidney, liver, skin and heart

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Ammonium sulfate precipitation + DEAE cellulose chromatography + Cross-absorption with immobilized human serum proteins.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200.
Dot blot Use at an assay dependent concentration.
ELISA 1/200.

Target

  • Function
    Type I collagen is a member of group I collagen (fibrillar forming collagen).
  • Tissue specificity
    Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • Involvement in disease
    Caffey disease
    Ehlers-Danlos syndrome, classic type
    Ehlers-Danlos syndrome 7A
    Osteogenesis imperfecta 1
    Osteogenesis imperfecta 2
    Osteogenesis imperfecta 3
    Osteogenesis imperfecta 4
    Osteoporosis
    A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • Sequence similarities
    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Domain
    The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
  • Post-translational
    modifications
    Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localization
    Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-1 type I collagen antibody
    • CO1A1_HUMAN antibody
    • COL1A1 antibody
    • COL2A1 antibody
    • COL3A1 antibody
    • COL4A1 antibody
    • COL5A1 antibody
    • Collagen alpha 1 chain antibody
    • Collagen alpha 1(I) chain antibody
    • Collagen alpha 1(II) chain antibody
    • Collagen alpha 1(III) chain antibody
    • Collagen alpha 1(IV) chain antibody
    • Collagen alpha 1(V) chain antibody
    • Collagen alpha-1(I) chain antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen II alpha 1 polypeptide antibody
    • Collagen IV alpha 1 polypeptide antibody
    • Collagen type I alpha 1 antibody
    • Collagen type II alpha 1 antibody
    • Collagen type III alpha 1 antibody
    • Collagen type IV alpha 1 antibody
    • Collagen type V alpha 1 antibody
    • EDS4A antibody
    see all

References for Anti-Collagen I + II + III + IV + V antibody (ab24117)

This product has been referenced in:
  • Price S  et al. The TrpA protein of Trichodesmium erythraeum IMS101 is a non-fibril-forming collagen and a component of the outer sheath. Microbiology 160:2148-56 (2014). Read more (PubMed: 25009239) »
  • Demidova-Rice TN  et al. Bioactive peptides derived from vascular endothelial cell extracellular matrices promote microvascular morphogenesis and wound healing in vitro. Wound Repair Regen 19:59-70 (2011). IP ; Human . Read more (PubMed: 21134032) »

See all 3 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab24117.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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