Anti-Collagen III antibody [1E7-D7/Col3] (ab23445)

Overview

  • Product nameAnti-Collagen III antibody [1E7-D7/Col3]See all Collagen III primary antibodies ...
  • Description
    Mouse monoclonal [1E7-D7/Col3] to Collagen III
  • Specificityab23445 is highly specific for type III Collagen. It has been shown to have no cross reactivity with type I and V Collagens. There is no evidence for cross reactivity with other connective tissue proteins (laminin, fibronectin, elastin).
  • Tested applicationsELISA, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Rat, Dog, Human, Pig, Kangaroo

    Does not react with

    Sheep, Cow
  • Immunogen

    Full length native protein (purified) corresponding to Human Collagen III.
    Database link: P02461

  • EpitopeThe epitope recognised by ab23445 is located approximately 100nm from the N terminal of the molecule.
  • Positive control
    • Frozen and unfixed human or kangaroo skin tissue slides.

Properties

Applications

Our Abpromise guarantee covers the use of ab23445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/500.
IHC-Fr Use at an assay dependent concentration. Note: Usually ab23445 is used in unfixed tissue slides. If fixation of tissue is required, acetone or ethanol is recommended.
WB Use at an assay dependent concentration. Predicted molecular weight: 134 kDa.

Target

  • FunctionCollagen type III occurs in most soft connective tissues along with type I collagen.
  • Involvement in diseaseDefects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
    Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
    Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
  • Sequence similaritiesBelongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications
    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CO3A1_HUMAN antibody
    • COL 3A1 antibody
    • COL3A1 antibody
    • Collagen alpha 1(III) chain antibody
    • Collagen alpha-1(III) chain antibody
    • Collagen III alpha 1 chain precursor antibody
    • Collagen III alpha 1 polypeptide antibody
    • Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibody
    • Collagen type III alpha 1 antibody
    • Collagen type III alpha antibody
    • EDS4A antibody
    • Ehlers Danlos syndrome type IV, autosomal dominant antibody
    • Fetal collagen antibody
    • Type III collagen antibody
    see all

Anti-Collagen III antibody [1E7-D7/Col3] images

  • Immunohistochemical analysis of frozen pig skin tissue, staining Collagen III with ab23445.

    Tissue was fixed with acetone and blocked with 10% serum for 10 minutes 20°C. Samples were incubated with primary antibody (1/500 in TBS, 0.02% Triton x-100, 2% BSA) for 16 hours at 4°C. An undiluted biotin-conjugated goat anti-mouse polyclonal IgG was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Paraffin-embedded sections) analysis of human bronchus tissue labelling Collagen III with ab23445.

References for Anti-Collagen III antibody [1E7-D7/Col3] (ab23445)

This product has been referenced in:

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Pig Tissue sections (pig skin frozen sections)
Specification pig skin frozen sections
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 20°C
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Verified customer

Submitted Jan 24 2013

Thank you foryour understanding and cooperation. The details provided enable us to closely monitor the quality of our products.

I hope the suggestions may proove useful with ab6310. It may be worth trying citrate antigen retreival with the P...

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Vielen Dank für Ihren Anruf.

Wie versprochen habe ich unsere Kollagen-Antikörper nach den von Ihnen genannten Kriteriengeprüft. Leider habe ich keinen perfekten Treffer gefunden, dafür aber vielleicht ein paar Kandidaten, ...

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