Anti-Collagen III antibody [1E7-D7/Col3] (ab23445)
Key features and details
- Mouse monoclonal [1E7-D7/Col3] to Collagen III
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Collagen III antibody [1E7-D7/Col3]
See all Collagen III primary antibodies -
Description
Mouse monoclonal [1E7-D7/Col3] to Collagen III -
Host species
Mouse -
Specificity
ab23445 is highly specific for type III Collagen. It has been shown to have no cross reactivity with type I and V Collagens. There is no evidence for cross reactivity with other connective tissue proteins (laminin, fibronectin, elastin). -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Does not react with: Sheep, Cow -
Immunogen
Full length native protein (purified) corresponding to Human Collagen III.
Database link: P02461 -
Epitope
The epitope recognised by ab23445 is located approximately 100nm from the N terminal of the molecule. -
Positive control
- Frozen and unfixed human or kangaroo skin tissue slides.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1E7-D7/Col3 -
Myeloma
NS1/1-Ag4-1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23445 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
Target
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Function
Collagen type III occurs in most soft connective tissues along with type I collagen. -
Involvement in disease
Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells. -
Sequence similarities
Belongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 VWFC domain. -
Post-translational
modificationsProline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 1281 Human
- Omim: 120180 Human
- SwissProt: P02461 Human
- Unigene: 443625 Human
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Alternative names
- Alpha 1 type III collagen antibody
- Alpha1 (III) collagen antibody
- CO3A1_HUMAN antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (22)
ab23445 has been referenced in 22 publications.
- Wei S et al. Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs. Mil Med Res 8:18 (2021). PubMed: 33685528
- Huang S et al. Myeloid differentiation 2 deficiency attenuates AngII-induced arterial vascular oxidative stress, inflammation, and remodeling. Aging (Albany NY) 13:4409-4427 (2021). PubMed: 33495414
- Chen J et al. Pathological changes of frozen shoulder in rat model and the therapeutic effect of PPAR-γ agonist. J Orthop Res 39:891-901 (2021). PubMed: 33222263
- Li Y et al. Exosomes derived from human adipose mesenchymal stem cells attenuate hypertrophic scar fibrosis by miR-192-5p/IL-17RA/Smad axis. Stem Cell Res Ther 12:221 (2021). PubMed: 33789737
- Li P et al. Consistency of pituitary adenomas: Amounts of collagen types I and III and the predictive value of T2WI MRI. Exp Ther Med 22:1255 (2021). PubMed: 34603523