The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesDot: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
IHC-Fr: 1/10 if FITC conjugated secondary antibody is used, or 1/20 for secondary-peroxidase conjugates.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCollagen type III occurs in most soft connective tissues along with type I collagen.
Involvement in diseaseDefects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity. Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas. Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
Sequence similaritiesBelongs to the fibrillar collagen family. Contains 1 fibrillar collagen NC1 domain. Contains 1 VWFC domain.
Post-translational modificationsProline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
Cellular localizationSecreted > extracellular space > extracellular matrix.
Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibody
Collagen type III alpha 1 antibody
Collagen type III alpha 1 chain antibody
Collagen type III alpha antibody
Collagen, fetal antibody
Ehlers Danlos syndrome type IV, autosomal dominant antibody
Fetal collagen antibody
Type III collagen antibody
Anti-Collagen III antibody images
Immunohistochemistry (Frozen sections) - Anti-Collagen III antibody (ab24119)Image courtesy of Daniel Holod-Betz by Abreview.
ab24119 staining Collagen III in pig epithelial tissue by Immunohistochemistry (Frozen sections). Tissue was fixed in acetone and then incubated with ab24119 at a 1/10 dilution for 1 hour at 25°C. The secondary used was ab98650 Goat F(ab')2 polyclonal Secondary Antibody to Mouse IgM - mu chain, used at a 1/1000 dilution. DAPI (Blue) stained for nuclei. Far Red stains for Collagen III.
References for Anti-Collagen III antibody (ab24119)
This product has been referenced in:
Müller NF et al. Trimeric autotransporter adhesin-dependent adherence of Bartonella henselae, Bartonella quintana, and Yersinia enterocolitica to matrix components and endothelial cells under static and dynamic flow conditions. Infect Immun79:2544-53 (2011).
Read more (PubMed: 21536788) »