Full length protein corresponding to Human Collagen IV.
In IHC, recognizes the extracellular – basement membranes.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 27th October 2017. The following lots are still unpurified and still in stock as of 27th October 2017- GR275641-7, GR275641-13, and GR275641-14. Lot numbers other than GR275641-7, GR275641-13, and GR275641-14 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.
Our Abpromise guarantee covers the use of ab86042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Use under non reducing condition. Predicted molecular weight: 160 kDa.|
|IHC-P||1/50 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||1/50 - 1/100.|
|ICC||1/50 - 1/100. Methanol fixed.|
|Flow Cyt||Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|