Full length native protein (purified) (Human).
Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.
Our Abpromise guarantee covers the use of ab6311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||1/25 - 1/50. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. PubMed: 18843029
We suggest using an ABC detection system.
|Dot blot||Use at an assay dependent concentration.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovary tissue sections labeling Collagen IV with ab6311 at 1/400 dilution. Tissue was blocked with 1.4% serum for 30 minutes. Heat mediated antigen retrieval was performed. An undiluted monoclonal alkaline phosphatase conjugated secondary antibody was used.
Blood vessels in whole ovarian section. Clear staining is seen in the vessel walls.
Immunocytochemistry/ Immunofluorescence analysis of human kidney cells labeling Collagen IV with ab6311 at 1/100 dilution. Cells were fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 25°C. A polyclonal rabbit anti-mouse Alexa Fluro® 488 was used as the secondary antibody at 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labeling Collagen IV with ab6311.
Immunofluorescence analysis of Human endothelial cells, staining Collagen IV with ab6311.
Cells were fixed with formaldehyde and permeabilized with acetone. Samples were incubated with primary antibody (0.5 µg/ml in diluent) for 18 hours at 4°C. An AlexaFluor®488-conjugated goat anti-mouse polyclonal IgG (5 µg/ml) (ab150113) was used as the secondary antibody.