Anti-Collagen Type V antibody [1E2-E4/Col5] (ab112551)

Overview

  • Product nameAnti-Collagen Type V antibody [1E2-E4/Col5]
    See all Collagen Type V primary antibodies
  • Description
    Mouse monoclonal [1E2-E4/Col5] to Collagen Type V
  • Specificityab112551 has been shown to have no cross-reactivity with type I, III and VI collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin).
  • Tested applicationsSuitable for: ELISA, IHC-P, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Sheep, Rabbit, Cow, Dog, Human, Pig, Kangaroo
    Does not react with: Mouse, Rat, Chicken, Guinea pig
  • Immunogen

    Acid-digested pepsin soluble Dog Type V Collagen.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 97% Phosphate Buffer, 2.9% Sodium chloride
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number1E2-E4/Col5
  • MyelomaNS1/1-Ag4-1
  • IsotypeIgG2a
  • Light chain typekappa

Applications

Our Abpromise guarantee covers the use of ab112551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/50.
ab112551 binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 184 kDa.
IHC-Fr Use at an assay dependent concentration.

Target

References for Anti-Collagen Type V antibody [1E2-E4/Col5] (ab112551)

ab112551 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Pig Tissue lysate - whole (Heart)
Specification Heart
Treatment 5 pigs are treated with pacemaker for 14 days (pm)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Jan 23 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"