Anti-Collagen V antibody [1.BB.755] (ab62426)


  • Product nameAnti-Collagen V antibody [1.BB.755]
    See all Collagen V primary antibodies
  • Description
    Mouse monoclonal [1.BB.755] to Collagen V
  • SpecificityThis antibody recognizes native human as well as heat denatured human collagen type V in RIA, ELISA and immunoblot assays. It does not react with other collagen types I, II, III and IV. Human plasma proteins do not interfere with binding to collagen.
  • Tested applicationsSuitable for: ELISA, RIA, IHC-Frmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Collagen Type V (Human)


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: Ascites
  • Concentration information loading...
  • Purification notesPurified by PEG6000 precipitation and Sepharose 6B gel filtration.
  • ClonalityMonoclonal
  • Clone number1.BB.755
  • IsotypeIgM
  • Research areas


Our Abpromise guarantee covers the use of ab62426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunohistochemistry (acetone fixed)
  • Application notesELISA: 1/1000. This antibody can also be used for quantitative sandwich ELISA to determine concentration of collagen type V in human fluids.
    IHC-Fr: Use at an assay dependent dilution.
    RIA: Use at an assay dependent dilution.

    Is unsuitable for IHC-P.

    For staining of tissue sections it is advised to use acetone or ethanol fixed cryostat sections, results of staining of paraffin embedded tissues, even after treatment with proteolytic enzymes or microwave, are less satisfactory. The antibody has to be diluted 1:20, if FITC- labeled secondary antibodies are used.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionType V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
    • Involvement in diseaseEhlers-Danlos syndrome 1
      Ehlers-Danlos syndrome 2
    • Sequence similaritiesBelongs to the fibrillar collagen family.
      Contains 1 fibrillar collagen NC1 domain.
      Contains 1 laminin G-like domain.
    • DomainThe C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
    • Post-translational
      Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
      Sulfated on 40% of tyrosines.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • AB collagen antibody
      • Alpha 1 type V collagen antibody
      • Alpha 2 type V collagen antibody
      • CO5A1_HUMAN antibody
      • COL5A1 antibody
      • Col5A2 antibody
      • COL5A2 protein antibody
      • Col5A3 antibody
      • Collagen alpha 1(V) chain antibody
      • Collagen alpha 2 (V) chain precursor antibody
      • Collagen alpha 2(V) chain antibody
      • Collagen alpha 3(V) chain antibody
      • Collagen alpha-1(V) chain antibody
      • Collagen fetal membrane A polypeptide antibody
      • Collagen type V alpha 1 antibody
      • Collagen type V alpha 2 antibody
      • Collagen type V alpha 3 antibody
      • Collagen V alpha 2 polypeptide antibody
      • CollagenV antibody
      • MGC105115 antibody
      • OTTHUMP00000064637 antibody
      • Pro alpha 1 type V collagen antibody
      • Pro alpha 3(V) collagen antibody
      • Procollagen alpha 2(V) antibody
      • Type V preprocollagen alpha 2 chain antibody
      see all

    References for Anti-Collagen V antibody [1.BB.755] (ab62426)

    ab62426 has not yet been referenced specifically in any publications.

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