Overview

  • Product name
  • Description
    Rabbit polyclonal to Collagen V
  • Specificity
    Cross-Reactivity (RIA%) Rat Collagen Type V 100% Rat Collagen Types I, III <0.1% Rat Elastin <0.1%
  • Tested applications
    Suitable for: ELISA, ICC/IF, RIAmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Collagen type V extracted and purified from rat skin.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Buffer: Not applicable.
    Preservative: None
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Ion exchange chromatography (DEAE-Trisacryl)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab21299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
ICC/IF
RIA
  • Application notes
    ELISA: Use at >1:100-1:200 dilution.
    IF: Use at an assay dependent dilution. In indirect IFA with fluorescein anti-rabbit IgG conjugate, use diluted at >1:40 on frozen rat tissue sections.
    (SP)RIA: Use at an assay dependent dilution.
    For immunostaining of extra or intracellular components in light microscopy. For the quantitation of Collagen Type V in biological fluids or in culture supernatants.
    Each laboratory should determine an optimum working titer for use in its particular application.
  • Target

    • Function
      Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
    • Involvement in disease
      Ehlers-Danlos syndrome 1
      Ehlers-Danlos syndrome 2
    • Sequence similarities
      Belongs to the fibrillar collagen family.
      Contains 1 fibrillar collagen NC1 domain.
      Contains 1 laminin G-like domain.
    • Domain
      The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
    • Post-translational
      modifications
      Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
      Sulfated on 40% of tyrosines.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • AB collagen antibody
      • Alpha 1 type V collagen antibody
      • Alpha 2 type V collagen antibody
      • CO5A1_HUMAN antibody
      • COL5A1 antibody
      • Col5A2 antibody
      • COL5A2 protein antibody
      • Col5A3 antibody
      • Collagen alpha 1(V) chain antibody
      • Collagen alpha 2 (V) chain precursor antibody
      • Collagen alpha 2(V) chain antibody
      • Collagen alpha 3(V) chain antibody
      • Collagen alpha-1(V) chain antibody
      • Collagen fetal membrane A polypeptide antibody
      • Collagen type V alpha 1 antibody
      • Collagen type V alpha 2 antibody
      • Collagen type V alpha 3 antibody
      • Collagen V alpha 2 polypeptide antibody
      • CollagenV antibody
      • MGC105115 antibody
      • OTTHUMP00000064637 antibody
      • Pro alpha 1 type V collagen antibody
      • Pro alpha 3(V) collagen antibody
      • Procollagen alpha 2(V) antibody
      • Type V preprocollagen alpha 2 chain antibody
      see all

    References

    ab21299 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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