The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at >1:100-1:200 dilution.
IF: Use at an assay dependent dilution. In indirect IFA with fluorescein anti-rabbit IgG conjugate, use diluted at >1:40 on frozen rat tissue sections.
(SP)RIA: Use at an assay dependent dilution.
For immunostaining of extra or intracellular components in light microscopy. For the quantitation of Collagen Type V in biological fluids or in culture supernatants.
Each laboratory should determine an optimum working titer for use in its particular application.
Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
Involvement in disease
Ehlers-Danlos syndrome 1 Ehlers-Danlos syndrome 2
Belongs to the fibrillar collagen family. Contains 1 fibrillar collagen NC1 domain. Contains 1 laminin G-like domain.
The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Sulfated on 40% of tyrosines.
Secreted > extracellular space > extracellular matrix.