• Product nameAnti-Collagen V antibody
    See all Collagen V primary antibodies
  • Description
    Rabbit polyclonal to Collagen V
  • SpecificityCross-Reactivity (RIA%) Rat Collagen Type V 100% Rat Collagen Types I, III <0.1% Rat Elastin <0.1%
  • Tested applicationsSuitable for: ELISA, ICC/IF, RIAmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Collagen type V extracted and purified from rat skin.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferBuffer: Not applicable.
    Preservative: None
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesIon exchange chromatography (DEAE-Trisacryl)
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab21299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: Use at >1:100-1:200 dilution.
    IF: Use at an assay dependent dilution. In indirect IFA with fluorescein anti-rabbit IgG conjugate, use diluted at >1:40 on frozen rat tissue sections.
    (SP)RIA: Use at an assay dependent dilution.
    For immunostaining of extra or intracellular components in light microscopy. For the quantitation of Collagen Type V in biological fluids or in culture supernatants.
    Each laboratory should determine an optimum working titer for use in its particular application.
  • Target

    • FunctionType V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
    • Involvement in diseaseEhlers-Danlos syndrome 1
      Ehlers-Danlos syndrome 2
    • Sequence similaritiesBelongs to the fibrillar collagen family.
      Contains 1 fibrillar collagen NC1 domain.
      Contains 1 laminin G-like domain.
    • DomainThe C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
    • Post-translational
      Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
      Sulfated on 40% of tyrosines.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • AB collagen antibody
      • Alpha 1 type V collagen antibody
      • Alpha 2 type V collagen antibody
      • CO5A1_HUMAN antibody
      • COL5A1 antibody
      • Col5A2 antibody
      • COL5A2 protein antibody
      • Col5A3 antibody
      • Collagen alpha 1(V) chain antibody
      • Collagen alpha 2 (V) chain precursor antibody
      • Collagen alpha 2(V) chain antibody
      • Collagen alpha 3(V) chain antibody
      • Collagen alpha-1(V) chain antibody
      • Collagen fetal membrane A polypeptide antibody
      • Collagen type V alpha 1 antibody
      • Collagen type V alpha 2 antibody
      • Collagen type V alpha 3 antibody
      • Collagen V alpha 2 polypeptide antibody
      • CollagenV antibody
      • MGC105115 antibody
      • OTTHUMP00000064637 antibody
      • Pro alpha 1 type V collagen antibody
      • Pro alpha 3(V) collagen antibody
      • Procollagen alpha 2(V) antibody
      • Type V preprocollagen alpha 2 chain antibody
      see all

    References for Anti-Collagen V antibody (ab21299)

    ab21299 has not yet been referenced specifically in any publications.

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