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Full length native protein (purified) corresponding to Collagen V aa 1-1745.
Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
Our Abpromise guarantee covers the use of ab7046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration. PubMed: 18385800|
|Sandwich ELISA||Use a concentration of 10 µg/ml. Can be used with a suitable biotinylated detection antibody such as Rabbit polyclonal to Collagen V (Biotin) (ab6582).|
|IHC-Fr||1/50 - 1/200.|
|IP||1/5000 - 1/10000.|
|WB||1/5000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 180 kDa). Native conditions are recommended.|
|IHC-P||1/50 - 1/200.|
ab7046 staining Collagen V in Dog skeletal muscle tissue by Immunohistochemistry (Frozen sections). The sections were fixed in Acetone. The primary antibody was diluted 1/100 in 10% Normal Goat Serum (ab7481) in PBS-T and incubated with the sample for 12 hours at 4°C. A Rhodamine-conjugated Goat anti-Rabbit IgG was used as the secondary antibody, diluted 1/200.
Immunohistochemical analysis of formalin-fixed paraffin-embedded human lung (left) and placenta (right) sections labelling collagen V with ab7046 at a dilution of 1/200 for 45 minutes at room temperature. An antigen retrival step was performed with 0.01 M sodium citrate buffer pH 6.0 at 100ºC for 20 mins.