Overview

  • Product nameAnti-Collagen VI antibody
    See all Collagen VI primary antibodies
  • Description
    Rabbit polyclonal to Collagen VI
  • SpecificityNegligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
  • Tested applicationsSuitable for: ICC/IF, ELISA, IHC-Fr, IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Cow, Dog, Human, Pig
    Predicted to work with: all Mammals
  • Immunogen

    Full length native protein (purified) corresponding to Human Collagen VI aa 1-1028. Collagen Type VI from human and bovine placenta.
    Database link: P12109

  • Positive control
    • HepG2. Pig retinal pigment epithelium. Human placenta.
  • General notes

    At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage bufferpH: 8.00
    Preservative: 0.01% Sodium azide
    Constituents: 0.44% Sodium chloride, 4.8% Sodium borate, 0.15% EDTA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesImmunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250 - 1/500.
ELISA 1/4000 - 1/8000.
IHC-Fr 1/50 - 1/200.
IP Use at an assay dependent concentration.
WB 1/5000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 108 kDa). This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.
IHC-P Use at an assay dependent concentration. PubMed: 18065394

Target

  • FunctionCollagen VI acts as a cell-binding protein.
  • Involvement in diseaseDefects in COL6A1 are a cause of Bethlem myopathy (BM) [MIM:158810]. BM is a rare autosomal dominant proximal myopathy characterized by early childhood onset (complete penetrance by the age of 5) and joint contractures most frequently affecting the elbows and ankles.
    Defects in COL6A1 are a cause of Ullrich congenital muscular dystrophy (UCMD) [MIM:254090]; also known as Ullrich scleroatonic muscular dystrophy. UCMD is an autosomal recessive congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy.
  • Sequence similaritiesBelongs to the type VI collagen family.
    Contains 3 VWFA domains.
  • Post-translational
    modifications
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha 1 (VI) chain (61 AA) antibody
    • CO6A1_HUMAN antibody
    • COL6A1 antibody
    • COL6A2 antibody
    • COL6A3 antibody
    • Collagen alpha 2(VI) chain antibody
    • Collagen alpha 3(VI) chain antibody
    • Collagen alpha-1(VI) chain antibody
    • Collagen type VI alpha 1 antibody
    • Collagen type VI alpha 2 antibody
    • Collagen type VI alpha 3 antibody
    • Collagen VI alpha 1 polypeptide antibody
    • Collagen VI alpha 2 polypeptide antibody
    • Collagen VI alpha 3 polypeptide antibody
    • CollagenVI antibody
    • Human mRNA for collagen VI alpha 2 C terminal globular domain antibody
    • OPLL antibody
    • PP3610 antibody
    see all

Anti-Collagen VI antibody images

  • Anti-Collagen VI antibody (ab6588) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 108 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of 10% buffered formalin-fixed swine lung tissue sections, labelling Collagen VI with ab6588 at a dilution of 1/400 incubated for 12 hours at 4°C. Antigen retrival was with 10mM sodium citrate buffer pH 6.0 (heat mediated). Blocking was with 5% serum incubated for 1 hour at 21°C. The secondary was a Donkey anti-Rabbit polyclonal Alexa Fluor® 647 conjugtate undiluted.

    See Abreview

  • ab6588 staining (green) Collagen VI in a primary cell culture from Mouse retinal pigment epithelium by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody 1/500 (1% goat serum, 0.1% TX100, 1XPBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat polyclonal to rabbit IgG (ab150077), dilution 1/500, was used as secondary antibody. Nuclei were counterstained with DAPI (blue).

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  • ab6588 staining Collagen VI in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde and an antigen retrieval step was performed using citrate buffer. Samples were then blocked with 10% serum for 30 minutes at 20°C and then incubated with ab6588 at a 1/50 dilution for 1 hour at 37°C. The secondary used was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal (ab150078), used at a 1/500 dilution.

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  • ab6588 staining Collagen VI in a primary cell culture from Pig retinal pigment epithelium by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. Samples were incubated with primary antibody 1/250 (1% goat serum, 0.1% TX100, 1XPBS)  for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat polyclonal to rabbit IgG, dilution 1/500, was used as secondary antibody. Nuclei were counterstained with DAPI (blue).

    See Abreview

  • ab6588 at 1/400 dilution staining Collagen VI in Human placenta (Top) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.

  • Immunohistochemical analysis of Formaldehyde fixed human colon tissue sections labelling Collagen VI with ab6588 at a dilution of 1/200. The secondary antibody used was HRP conjugated rabbit polyclonal secondary. Antigen retrieval was heat mediated. 

    See Abreview

References for Anti-Collagen VI antibody (ab6588)

This product has been referenced in:
  • Kristofik N  et al. Impaired von Willebrand factor adhesion and platelet response in thrombospondin-2 knockout mice. Blood 128:1642-50 (2016). Read more (PubMed: 27471233) »
  • Okawa S  et al. Lipopolysaccharide induces expression of collagen VI in the rat lung. J Toxicol Pathol 28:37-41 (2015). WB, IHC-P ; Rat . Read more (PubMed: 26023260) »

See all 24 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Artery)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization No
Specification Artery
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% normal buffered formalin
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Submitted Sep 07 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (artery)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization No
Specification artery
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% normal buffered formalin
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Submitted Sep 01 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (artery)
Permeabilization No
Specification artery
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative Paraformaldehyde
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Submitted Jan 29 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (bone)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization No
Specification bone
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% normal buffered formalin
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Submitted Jan 28 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium citrate buffer pH 6
Permeabilization No
Specification lung
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% buffered formalin
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Submitted Sep 30 2015

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (muscle)
Permeabilization No
Specification muscle
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative Acetone
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Submitted Sep 25 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (breast tumor xenograft)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH 6
Permeabilization No
Specification breast tumor xenograft
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% buffered formalin
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Submitted Aug 24 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (pancreatic tumor)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH 6
Permeabilization No
Specification pancreatic tumor
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% buffered formalin
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Submitted Aug 11 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.05% typsin
Sample Human Tissue sections (human whole fat tissue)
Specification human whole fat tissue
Permeabilization No
Fixative Formaldehyde
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Submitted Mar 18 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (Sucutanous adipose tissue)
Specification Sucutanous adipose tissue
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 8% · Temperature: 20°C
Username

Mr. Prabhat Khanal

Verified customer

Submitted Jan 02 2013

1-10 of 43 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"