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Collagen Type VI from adult human and bovine tissues
Our Abpromise guarantee covers the use of ab6583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/4000 - 1/8000.|
|IHC-Fr||1/50 - 1/200.|
|IP||Use at an assay dependent concentration.|
|WB||1/5000 - 1/10000. This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
ab6583 staining Collagen VI (red) in Bovine bone marrow stromal cells (BMSCs) and articular chondrocytes (ACs) by ICC/IF (Immunocytochemistry/immunofluorescence). Sections were fixed with formalin and blocked with 5% BSA for 30 minutes. Samples were incubated with primary antibody (10 μg/ml). An Alexa Fluor®-conjugated anti-rabbit IgG was used as the secondary antibody. Decorin - green, DNA - blue.