Overview

  • Product name
    Anti-Collagen VII antibody
    See all Collagen VII primary antibodies
  • Description
    Rabbit polyclonal to Collagen VII
  • Tested applications
    Suitable for: WB, IP, ELISA, Flow Cyt, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Common marmoset
  • Immunogen

    Full length native protein (purified) corresponding to Human Collagen VII.

Properties

Applications

Our Abpromise guarantee covers the use of ab93350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ELISA
Flow Cyt
ICC/IF
IHC-P
  • Application notes
    ELISA: Use at an assay dependent dilution.
    EIA: Use at an assay dependent dilution.
    Flow Cyt: Use at an assay dependent dilution.
    ICC/IF: Use at a concentration of 2.5 - 10 µg/ml.
    IP: Use at a concentration of 2.5 - 10 µg/ml.
    WB: Use at a concentration of 0.2 - 0.8 µg/ml. Predicted molecular weight: 295 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
    • Involvement in disease
      Epidermolysis bullosa acquisita (EBA) is an autoimmune acquired blistering skin disease resulting from autoantibodies to type VII collagen.
      Epidermolysis bullosa dystrophica, autosomal dominant
      Epidermolysis bullosa dystrophica, autosomal recessive
      Epidermolysis bullosa dystrophica, Pasini type
      Epidermolysis bullosa dystrophica, Hallopeau-Siemens type
      Transient bullous dermolysis of the newborn
      Epidermolysis bullosa dystrophica, pretibial type
      Epidermolysis bullosa dystrophica, Bart type
      Epidermolysis bullosa pruriginosa
      Nail disorder, non-syndromic congenital, 8
      Epidermolysis bullosa dystrophica, with subcorneal cleavage
    • Sequence similarities
      Contains 1 BPTI/Kunitz inhibitor domain.
      Contains 9 fibronectin type-III domains.
      Contains 2 VWFA domains.
    • Post-translational
      modifications
      Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha 1 type VII collagen antibody
      • CO7A1_HUMAN antibody
      • Col7a1 antibody
      • Collagen alpha 1(VII) chain antibody
      • Collagen alpha-1(VII) chain antibody
      • Collagen type VII alpha 1 antibody
      • Collagen VII alpha 1 polypeptide antibody
      • CollagenVII antibody
      • EBD 1 antibody
      • EBD1 antibody
      • EBDCT antibody
      • EBR 1 antibody
      • EBR1 antibody
      • LC collagen antibody
      • Long chain collagen antibody
      • Long-chain collagen antibody
      see all

    Anti-Collagen VII antibody images

    • Immunohistochemical staining of Collagen VII in human skin tissue sections with ab93350. Samples were fixed with formaldehyde and enzymatic antigen retrieval was performed with Proteinase K. Tissue was blocked with 1% BSA for 10 minutes at 21°C before ab93350 was added (1/250) for 2 hours at 21°C. A biotin conjugated goat anti-rabbit secondary was used at 1/250.

      See Abreview

    References for Anti-Collagen VII antibody (ab93350)

    This product has been referenced in:
    • Gouveia RM  et al. Controlling the 3D architecture of Self-Lifting Auto-generated Tissue Equivalents (SLATEs) for optimized corneal graft composition and stability. Biomaterials 121:205-219 (2017). IF ; Human . Read more (PubMed: 28092777) »

    See 1 Publication for this product

    Product Wall

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (skin)
    Permeabilization
    No
    Specification
    skin
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Acetone
    Username

    Abcam user community

    Verified customer

    Submitted Jul 12 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: Proteinase K
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Aug 19 2014

    Thank you for your inquiry.

    I heard back from the lab that our anti-Collagen VII antibody is specific only for helical domains (collagen VII fragments). The non-collagen fragments obtained after pepsin digestion are broken and cannot be detec...

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    Thank you for contacting us.
    Both antibodies were run under reducing and denaturing conditions.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use o...

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    Thank you for your enquiry.

    I can confirm that any publications that we are aware of will be listed on the datasheet. We have a dedicated team who spend time reviewing the literature to find articles that have used our products. However, in ma...

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    Thank you for contacting us. The BLAST results show 84% homology between the full-length human (immunogen) and mouse and is likely to work. If you are interested in this product we do have a testing discount program which acts as a buy one get one free...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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