Overview

  • Product nameAnti-Collagen VII antibody [LH7.2]
    See all Collagen VII primary antibodies
  • Description
    Mouse monoclonal [LH7.2] to Collagen VII
  • Tested applicationsSuitable for: WB, ELISA, IHC-Fr, ICC/IF, ICC, Dot blot, IHC-Pmore details
  • Species reactivity
    Reacts with: Sheep, Goat, Horse, Guinea pig, Cow, Human
  • Immunogen

    Full length native protein (purified) (Human). Insoluble fractions prepared from neonatal foreskin epidermal cells.

  • EpitopeRecognizes an epitope located on collagenase digested Type VII collagen (150kD), i.e. the non-helical carboxy terminal region of the Type VII collagen dimer. Immunoelectron microscopy shows antibody binding localised at the inferior border of the lamina densa.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR158908. Lot numbers higher than GR158908 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.”

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.097% Sodium azide
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberLH7.2
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-Fr
ICC/IF
ICC
Dot blot
IHC-P
  • Application notesDot: Use at an assay dependant dilution.
    ELISA: Use at an assay dependant dilution.
    ICC: Use at an assay dependant dilution.
    IF: 1/1000. (human or other mammalian frozen sections)
    IHC-Fr: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionStratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
    • Involvement in diseaseEpidermolysis bullosa acquisita (EBA) is an autoimmune acquired blistering skin disease resulting from autoantibodies to type VII collagen.
      Epidermolysis bullosa dystrophica, autosomal dominant
      Epidermolysis bullosa dystrophica, autosomal recessive
      Epidermolysis bullosa dystrophica, Pasini type
      Epidermolysis bullosa dystrophica, Hallopeau-Siemens type
      Transient bullous dermolysis of the newborn
      Epidermolysis bullosa dystrophica, pretibial type
      Epidermolysis bullosa dystrophica, Bart type
      Epidermolysis bullosa pruriginosa
      Nail disorder, non-syndromic congenital, 8
      Epidermolysis bullosa dystrophica, with subcorneal cleavage
    • Sequence similaritiesContains 1 BPTI/Kunitz inhibitor domain.
      Contains 9 fibronectin type-III domains.
      Contains 2 VWFA domains.
    • Post-translational
      modifications
      Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha 1 type VII collagen antibody
      • CO7A1_HUMAN antibody
      • Col7a1 antibody
      • Collagen alpha 1(VII) chain antibody
      • Collagen alpha-1(VII) chain antibody
      • Collagen type VII alpha 1 antibody
      • Collagen VII alpha 1 polypeptide antibody
      • CollagenVII antibody
      • EBD 1 antibody
      • EBD1 antibody
      • EBDCT antibody
      • EBR 1 antibody
      • EBR1 antibody
      • LC collagen antibody
      • Long chain collagen antibody
      • Long-chain collagen antibody
      see all

    Anti-Collagen VII antibody [LH7.2] images

    References for Anti-Collagen VII antibody [LH7.2] (ab6312)

    This product has been referenced in:
    • Ponsioen TL  et al. Human retinal Müller cells synthesize collagens of the vitreous and vitreoretinal interface in vitro. Mol Vis 14:652-60 (2008). ICC/IF ; Human . Read more (PubMed: 18385800) »

    See 1 Publication for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (Skin)
    Permeabilization No
    Specification Skin
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative Acetone
    Username

    Abcam user community

    Verified customer

    Submitted Jul 11 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
    Sample Human Tissue sections (Skin)
    Specification Skin
    Permeabilization No
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Aug 19 2014

    Thank you for contacting us.
    Both antibodies were run under reducing and denaturing conditions.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use o...

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    Thank you for contacting us.

    I have attached the reference paper which the lab cited as researchers having used this antibody.
    As far as I could see they did do the collagenase treatment and obtained the 150 kDa band. This is most likel...

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    I am very pleased to hear you would like to accept our offer and test ab6312 and ab80790in mouse. This code will give you: 1 free PRIMARY ANTIBODY OR COST OF THE PRODUCT OFF OF ORDER before the expiration date. To redeem this offer, please s...

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    Thank you very much for your enquiry. All the information that we have regarding these antibodies is located on the online datasheets, please refer to them for more information. Here are the expected band sizes for these antibodies: ab7045 - 21.6 kDa ...

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    The immunogen used to raise this antibody was insoluble fractions prepared from neonatal foreskin epidermal cells. Unfortunately, I have not been able to source a supplier for the recombinant protein.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"