Anti-Collagen VIII alpha 1 antibody (ab100988)

Overview

  • Product nameAnti-Collagen VIII alpha 1 antibody
    See all Collagen VIII alpha 1 primary antibodies
  • Description
    Rabbit polyclonal to Collagen VIII alpha 1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Chicken, Pig, Macaque Monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 600 - 700 of Human Collagen VIII alpha 1.

  • Positive control
    • This antibody gave a positive signal in the following Human tissue lysates: Heart; Kidney; Spleen; Liver; Lung.

Properties

Applications

Our Abpromise guarantee covers the use of ab100988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 73 kDa).

Target

  • FunctionMacromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in artherogenesis.
    Vastatin, the C-terminal fragment comprising the NC1 domain, inhibits aortic endothelial cell proliferation and causes cell apoptosis.
  • Tissue specificityExpressed primarily in the subendothelium of large blood vessels. Also expressed in arterioles and venules in muscle, heart, kidney, spleen, umbilical cord, liver and lung and is also found in connective tissue layers around hair follicles, around nerve bundles in muscle, in the dura of the optic nerve, in cornea and sclera, and in the perichondrium of cartilaginous tissues. In the kidney, expressed in mesangial cells, glomerular endothelial cells, and tubular epithelial cells. Also expressed in mast cells, and in astrocytes during the repair process. Expressed in Descemet's membrane. Specifically expressed in peritoneal fibroblasts and mesothelial cells.
  • Sequence similaritiesContains 1 C1q domain.
  • Post-translational
    modifications
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    Proteolytically cleaved by neutrophil elastase, in vitro. Proteolytic processing produces the C-terminal NC1 domain fragment, vastatin.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C3orf7 antibody
    • CO8A1_HUMAN antibody
    • COL8A1 antibody
    • Collagen alpha 1(VIII) chain [Precursor] antibody
    • Endothelial collagen antibody
    • MGC9568 antibody
    • Vastatin antibody
    see all

Anti-Collagen VIII alpha 1 antibody images

  • All lanes : Anti-Collagen VIII alpha 1 antibody (ab100988) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)
    Lane 4 : Human liver tissue lysate - total protein (ab29889)
    Lane 5 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 73 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References for Anti-Collagen VIII alpha 1 antibody (ab100988)

ab100988 has not yet been referenced specifically in any publications.

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