Overview

  • Product name
  • Description
    Rabbit polyclonal to Collagen X
  • Specificity
    ab58632 recognizes type X collagen. Exhibits slight cross-reactivity with fibronectin and type II and type IX collagen. Does not cross-react with type I, type III, or type XI collagen.
  • Tested applications
    Suitable for: ICC/IF, WB, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Cow, Human
  • Immunogen

    Full length native protein (purified) Type X collagen from rat chondrosarcoma cells

Properties

Applications

Our Abpromise guarantee covers the use of ab58632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/100 - 1/300.
IP 1/20 - 1/40.
IHC-Fr Use at an assay dependent concentration. Enzymatic or heat (41°C) pretreatment is recommended.

Target

  • Function
    Type X collagen is a product of hyperthrophic chondrotocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
  • Involvement in disease
    Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD) [MIM:156500]. SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity.
  • Sequence similarities
    Contains 1 C1q domain.
  • Post-translational
    modifications
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • COAA1_HUMAN antibody
    • Col10a 1 antibody
    • COL10A1 antibody
    • Collagen alpha 1(X) chain antibody
    • Collagen alpha-1(X) chain antibody
    • Collagen type X alpha 1 (Schmid metaphyseal chondrodysplasia) antibody
    • Collagen type X alpha 1 antibody
    • Collagen X alpha 1 polypeptide antibody
    • CollagenX antibody
    • fa66d11 antibody
    • fb10c08 antibody
    • OTTHUMP00000040411 antibody
    • Procollagen type X alpha 1 antibody
    • Schmid metaphyseal chondrodysplasia antibody
    • wu:fa66d11 antibody
    • wu:fb10c08 antibody
    see all

Images

  • Immunofluorescence analysis of mouse bone marrow stromal cells, staining Collagen X with ab58632.

    Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 before incubating with primary antibody (1/50) overnight. An AlexaFluor®488-conjugated anti-rabbit IgG (1/500) was used as the secondary antibody.
  • Anti-Collagen X antibody (ab58632) at 1/500 dilution + HT 1080 (Human fibrosarcoma) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 66 + 64 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,50 kDa,98 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 150 secondsThe band observed at 64 kDa could potentially be a cleaved form of Collagen X due to the presence of a 18 amino acid signal peptide.

References

This product has been referenced in:
  • Tidd N  et al. Minicircle Mediated Gene Delivery to Canine and Equine Mesenchymal Stem Cells. Int J Mol Sci 18:N/A (2017). IHC ; Horse . Read more (PubMed: 28417917) »
  • Xu T  et al. Hedgehog signal expression in articular cartilage of rat temporomandibular joint and association with adjuvant-induced osteoarthritis. J Oral Pathol Med 46:284-291 (2017). Read more (PubMed: 27646982) »

See all 48 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (cartilage)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: proteinase K 10ug/ml
Permeabilization
No
Specification
cartilage
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 24°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 16 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Bone Marrow)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
No
Specification
Bone Marrow
Blocking step
Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
10% NBF
Username

Premier Laboratory

Verified customer

Submitted May 04 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Knee)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
No
Specification
Knee
Blocking step
Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
10%NBF fix, 10% Formic Acid decalcification
Username

Premier Laboratory

Verified customer

Submitted May 04 2016

Antibody ab58632 is our recommendation, given the number of references that have used it for IHC of FFPE tissue. Here is a link to the list of references, annotated "IHC-P" for paraffin.

http://www.abcam.com/collagen-x-antibody-ab58632-referen...

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Thank you for contacting us. The anti-Collagen X antibody ab58632 is a whole serum antibody and the concentration has not been determined. Generally concentrations are only relevant for purified antibodies, since products from ascites or whole serum ma...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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Thank you for contacting us and for your interest in our products.

I am sorry to confirm that as far as we are aware, ab58632 has never been tested with samples from sheep. All tested and guaranteedspecies cross-reactivity information is sta...

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Thank you for contacting us.

The antibody is whole, unpurified antiserum, and the antibody concentration is not determined. Dilutions for some common applications are suggested on the datasheet. The appropriate isotype control for this antib...

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Thank you for your inquiry. Please find a list of possible positive controls below: ab2426: skin, lymph nodes Click here (or use the following: http://www.abcam.com/index.html?datasheet=2426). ab3773: cartilage samples Click here (or use the followin...

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Thank you for your call yesterday and for your patience while I have been in touch with the lab. For ab58632, we recommend using the antibody at 1:50-1:100 and incubating overnight. The sections were treated with 0.25% trypsin in 1 mM EDTA for...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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