Purified pig Collagen type X.
Our Abpromise guarantee covers the use of ab49945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 66 kDa.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20406908|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ostochondroma tissue labelling Collagen X with ab49945 at a dilution of 1/1000.
ICC/IF of HT1080 human fibrosarcoma cells labelling Collagen X with ab49945 at 1/1000 dilution. The cells were methanol acetone fixed. Secondary antibody was a Cy3 anti mouse IgG. Nuclei are stained with DAPI.