Overview

  • Product name
    Anti-Collagen X antibody [COL-10]
    See all Collagen X primary antibodies
  • Description
    Mouse monoclonal [COL-10] to Collagen X
  • Tested applications
    Suitable for: ELISA, Dot blot, WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Rabbit, Human, Pig, Deer
  • Immunogen

    Purified pig Collagen type X.

  • Epitope
    Hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with porcine collagen type X.
  • Positive control
    • IHC-P: Enzyme treated sections of decalcified human osteochondroma.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.097% Sodium azide
    Constituent: 99% Ascites
  • Concentration information loading...
  • Purity
    Ascites
  • Clonality
    Monoclonal
  • Clone number
    COL-10
  • Isotype
    IgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 66 kDa.
IHC-P 1/1000.
IHC-Fr Use at an assay dependent concentration. PubMed: 20406908
ICC/IF 1/1000.

Target

  • Function
    Type X collagen is a product of hyperthrophic chondrotocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
  • Involvement in disease
    Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD) [MIM:156500]. SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity.
  • Sequence similarities
    Contains 1 C1q domain.
  • Post-translational
    modifications
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • COAA1_HUMAN antibody
    • Col10a 1 antibody
    • COL10A1 antibody
    • Collagen alpha 1(X) chain antibody
    • Collagen alpha-1(X) chain antibody
    • Collagen type X alpha 1 (Schmid metaphyseal chondrodysplasia) antibody
    • Collagen type X alpha 1 antibody
    • Collagen X alpha 1 polypeptide antibody
    • CollagenX antibody
    • fa66d11 antibody
    • fb10c08 antibody
    • OTTHUMP00000040411 antibody
    • Procollagen type X alpha 1 antibody
    • Schmid metaphyseal chondrodysplasia antibody
    • wu:fa66d11 antibody
    • wu:fb10c08 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ostochondroma tissue labelling Collagen X with ab49945 at a dilution of 1/1000.

  • ICC/IF of HT1080 human fibrosarcoma cells labelling Collagen X with ab49945 at 1/1000 dilution. The cells were methanol acetone fixed. Secondary antibody was a Cy3 anti mouse IgG. Nuclei are stained with DAPI.

References

This product has been referenced in:
  • He A  et al. Repair of osteochondral defects with in vitro engineered cartilage based on autologous bone marrow stromal cells in a swine model. Sci Rep 7:40489 (2017). IHC-P ; Pig . Read more (PubMed: 28084417) »
  • Rocio Servin-Vences M  et al. Direct measurement of TRPV4 and PIEZO1 activity reveals multiple mechanotransduction pathways in chondrocytes. Elife 6:N/A (2017). ICC/IF ; Human . Read more (PubMed: 28135189) »

See all 17 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Growth plate cartilage)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: 700U/ml hyaluronidase
Permeabilization
Yes - 0.2% Triton X 100 in TBS
Specification
Growth plate cartilage
Blocking step
10% goat serum + 1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde
Username

Mr. Chris Erickson

Verified customer

Submitted Jan 06 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Cartilage)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: hyaluronidase
Permeabilization
Yes - triton X-100
Specification
Cartilage
Blocking step
BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 28 2015

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (femur (embryonic))
Permeabilization
Yes - 40%OCT
Specification
femur (embryonic)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 04 2015

Thank you for contacting us.

I am sorry we do not have an anybody to the central region of collagen X protein.

Unfortunately the epitope of ab4995 has not been determined so we are unable to confirm which part of protein this ab bind...

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DISCOUNT CODE: xxx Expiration date: xxx I am very pleased to hear you would like to accept our offer and test ab49945 on rabbit. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abre...

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I am very pleased to hear you would like to accept our offer and test ab49945 in rabbit. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rabbit and include this code in the...

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Thank you for your enquiry. According to our files, the samples were treated first with 0.2% hyaluronidase for 1 hour at 37 deg C and then treated with 0.1% protease for 9 minutes at 37 deg C. I hope this information will be useful. Should you r...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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