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Purified pig Collagen type X.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab58632 as a replacement.
Our Abpromise guarantee covers the use of ab49945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 66 kDa.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20406908|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ostochondroma tissue labelling Collagen X with ab49945 at a dilution of 1/1000.
ICC/IF of HT1080 human fibrosarcoma cells labelling Collagen X with ab49945 at 1/1000 dilution. The cells were methanol acetone fixed. Secondary antibody was a Cy3 anti mouse IgG. Nuclei are stained with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"