Anti-Collagen XVII antibody (ab28440)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab28440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
WB 1/500. Predicted molecular weight: 180 kDa.
ICC/IF 1/100.
ICC 1/100.

Target

  • RelevanceUnlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in the gene coding for collagen XVII are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD 1, is generated by proteolytic processing of the full length form. Two transcript variants, one resulting from alternative splicing in the 3' UTR, have been identified for this gene.
  • Cellular localizationCell junction, hemidesmosome. Membrane; Single-pass type II membrane protein. Note=Localized along the plasma membrane of the hemidesmosome. 120 kDa linear IgA disease antigen and 97 kDa linear IgA disease antigen: Secreted, extracellular space, extracellular matrix, basement membrane.
  • Database links
  • Alternative names
    • 180 kDa bullous pemphigoid antigen 2 antibody
    • Alpha 1 type XVII collagen antibody
    • BA16H23.2 antibody
    • BP 180 antibody
    • BP180 antibody
    • BPA 2 antibody
    • BPAG 2 antibody
    • BPAG2 antibody
    • Bullous pemphigoid antigen 2 antibody
    • COL17A1 antibody
    • Collagen 17 antibody
    • Collagen alpha 1 XVII chain antibody
    • Collagen alpha 1(XVII) chain antibody
    • Collagen alpha1 XVII chain antibody
    • Collagen type XVII alpha 1 antibody
    • Collagen XVII alpha 1 polypeptide antibody
    • CollagenXVII antibody
    • Epidermolysis bullosa junctional localisata variant antibody
    • FLJ60881 antibody
    • KIAA0204 antibody
    • LAD 1 antibody
    • LAD1 antibody
    see all

References for Anti-Collagen XVII antibody (ab28440)

ab28440 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HaCaT-ras II4 organotypic culture)
Specification HaCaT-ras II4 organotypic culture
Fixative Formaldehyde
Permeabilization Yes - 0.2% Triton-X100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
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Submitted Sep 28 2012

Vielen Dank für Ihre Antwort.

Der Code ist an einen spezifischen Antikörper gebunden und kann nicht für ab79878 verwendet werden. ich werde Ihnen aber gerne einen neuen Code für ab79878 ausstellen.

Ich möcht...

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DISCOUNT CODE: 100%00000
Ablaufdatum: 03. Oktober 2012

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab28440 in FACS zu testen, annehmen möchten.

Das Immunogen liegt zwischen Aminosäure 507-548von humanem Co...

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Thank you very much for your interest in our products.

We currently do not have an Anti-Collagen XVII antibody that is tested and guaranteed for IHC-Fr and flow cytometry.

I am not sure how extracellular collagen can be detected in ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"