Anti-Collagen XVII antibody [NC16A-3] (ab79878)

Overview

  • Product nameAnti-Collagen XVII antibody [NC16A-3]
    See all Collagen XVII primary antibodies
  • Description
    Mouse monoclonal [NC16A-3] to Collagen XVII
  • Tested applicationsSuitable for: IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified recombinant NC16a-domain of human collagen XVII

  • Positive control
    • Human normal skin tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberNC16A-3
  • IsotypeIgG1
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab79878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/40. For 60 minutes at room temperature for Biotin based detection systems. May require polymer detection. For optimal staining, the sections should be pretreated with an antigen unmasking solution.
ELISA Use at an assay dependent concentration.

Target

  • RelevanceUnlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in the gene coding for collagen XVII are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD 1, is generated by proteolytic processing of the full length form. Two transcript variants, one resulting from alternative splicing in the 3' UTR, have been identified for this gene.
  • Cellular localizationCell junction, hemidesmosome. Membrane; Single-pass type II membrane protein. Note=Localized along the plasma membrane of the hemidesmosome. 120 kDa linear IgA disease antigen and 97 kDa linear IgA disease antigen: Secreted, extracellular space, extracellular matrix, basement membrane.
  • Database links
  • Alternative names
    • 180 kDa bullous pemphigoid antigen 2 antibody
    • Alpha 1 type XVII collagen antibody
    • BA16H23.2 antibody
    • BP 180 antibody
    • BP180 antibody
    • BPA 2 antibody
    • BPAG 2 antibody
    • BPAG2 antibody
    • Bullous pemphigoid antigen 2 antibody
    • COL17A1 antibody
    • Collagen 17 antibody
    • Collagen alpha 1 XVII chain antibody
    • Collagen alpha 1(XVII) chain antibody
    • Collagen alpha1 XVII chain antibody
    • Collagen type XVII alpha 1 antibody
    • Collagen XVII alpha 1 polypeptide antibody
    • CollagenXVII antibody
    • Epidermolysis bullosa junctional localisata variant antibody
    • FLJ60881 antibody
    • KIAA0204 antibody
    • LAD 1 antibody
    • LAD1 antibody
    see all

References for Anti-Collagen XVII antibody [NC16A-3] (ab79878)

ab79878 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Purified protein (purified protein)
Specification purified protein
Blocking step roch blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Type Direct
Username

Abcam user community

Verified customer

Submitted Mar 01 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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I have now received a reply from lab; they unfortunately have same lot in stock. They have agreed to retest the lot, I will share the results as soon as I receive from them.

This antibody is stored at 4C and we guarantee its viability for 1 ye...

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I am having discussion with lab regarding the new lot. I will get back to you soon.

Many thanks for having patience!

Thank you very much for your email.

You can order the antibody with usual ordering process from our website. We have two lots GR85966-1, GR85966-2 available in stock; these are however from same mother stock. Is this lot number different than...

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Thank you very much for your email.

I am sorry we could not find a reason, why the particularantibodyvial isactive than expected. All the lots we produced passed the strict quality control criteria however we can not deny small variations due ...

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I'm happy to help! Best of luck in your research. Feel free to contact me if you need anything further.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team...

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Thanks for your email.

I am sorry for any confusion here. I believe we were just waiting to find out which antibody you would like as the free replacement for the faulty vial of ab79878 you received in February of this year. If you wish I am ...

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Vielen Dank für Ihre Antwort.

Der Code ist an einen spezifischen Antikörper gebunden und kann nicht für ab79878 verwendet werden. ich werde Ihnen aber gerne einen neuen Code für ab79878 ausstellen.

Ich möcht...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"