Anti-Collagenase antibody - BSA and Azide free (Biotin) (ab182881)

Overview

  • Product name
    Anti-Collagenase antibody - BSA and Azide free (Biotin)
    See all Collagenase primary antibodies
  • Description
    Rabbit polyclonal to Collagenase - BSA and Azide free (Biotin)
  • Host species
    Rabbit
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: IHC-P, ELISA, ICC, WB, Dot blotmore details
  • Immunogen

    Full length native protein (purified) corresponding to Collagenase. Purified from Clostridium histolyticum.

Properties

Applications

Our Abpromise guarantee covers the use of ab182881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/5000 - 1/100000.
ELISA 1/5000 - 1/100000.
ICC 1/5000 - 1/100000.
WB 1/5000 - 1/100000.
Dot blot 1/5000 - 1/100000.

Target

  • Relevance
    Function: Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. Domain: There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases). The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. PTM: Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. Similarity: Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.
  • Cellular localization
    Secreted, extracellular space, extracellular matrix
  • Alternative names
    • CLG antibody
    • Fibroblast collagenase antibody
    • matrix metallopeptidase 1 (interstitial collagenase) antibody
    • matrix metallopeptidase 1 antibody
    • MMP-1 antibody
    see all

References

ab182881 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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