Overview

  • Product nameAnti-COLQ antibody
    See all COLQ primary antibodies
  • Description
    Rabbit polyclonal to COLQ
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow
  • Immunogen

    A region within synthetic peptide: MVVLNPMTLG IYLQLFFLSI VSQPTFINSV LPISAALPSL DQKKRGGHKA, corresponding to N terminal amino acids 1-50 of Human COLQ.

  • Positive control
    • HepG2 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1/312500.

Target

  • FunctionAnchors the catalytic subunits of asymmetric AChE to the synaptic basal lamina.
  • Tissue specificityFound at the end plate of skeletal muscle.
  • Involvement in diseaseDefects in COLQ are the cause of congenital myasthenic syndrome Engel type (CMSE) [MIM:603034]; also known as end-plate acetylcholinesterase deficiency or congenital myasthenic syndrome type IC (CMS-IC). CMSE is a rare autosomal recessive congenital myasthenic syndrome characterized by onset during childhood, generalized weakness, abnormal fatigability on exertion, refrectoriness to acetylcholinesterase drugs, decremental electromyographic response and morphological abnormalities of the neuromuscular junctions.
  • Sequence similaritiesBelongs to the COLQ family.
    Contains 2 collagen-like domains.
  • DomainThe proline-rich attachment domain (PRAD) binds the AChE catalytic subunits.
  • Post-translational
    modifications
    The triple-helical tail is stabilized by disulfide bonds at each end.
  • Cellular localizationCell junction > synapse.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acetylcholinesterase collagen-like tail subunit isoform I antibody
    • Acetylcholinesterase collagenic tail peptide antibody
    • Acetylcholinesterase collagenic tail peptide precursor antibody
    • Acetylcholinesterase-associated collagen antibody
    • AChE Q subunit antibody
    • asymmetric acetylcholinesterase antibody
    • Collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase antibody
    • Colq antibody
    • COLQ_HUMAN antibody
    • EAD antibody
    • OTTHUMP00000209566 antibody
    • OTTHUMP00000209567 antibody
    • single strand of homotrimeric collagen-like tail subunit of asymmetric acetylcholinesterase antibody
    see all

Anti-COLQ antibody images

  • Anti-COLQ antibody (ab49190) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution
    Developed using the ECL technique

    Predicted band size : 48 kDa
    Observed band size : 48 kDa

References for Anti-COLQ antibody (ab49190)

ab49190 has not yet been referenced specifically in any publications.

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