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Rabbit polyclonal to COMMD9
antigen sequence, corresponding to amino acids 41-171 of Human COMMD9.
- COMMD9-transfected HEK293T lysate; Human small intestine tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
Contains 1 COMM domain.
Information by UniProt
- COMD9_HUMAN antibody
- COMM domain containing 9 antibody
- COMM domain-containing protein 9 antibody
Immunocytochemistry/ Immunofluorescence - Anti-COMMD9 antibody (ab121303)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and golgi apparatus. Recommended concentration of ab121303 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-COMMD9 antibody (ab121303)
All lanes : Anti-COMMD9 antibody (ab121303) at 1/250 dilution
Lane 1 : vector only transfected HEK293T lysate
Lane 2 : COMMD9 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells
Developed using the ECL technique10-20% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMMD9 antibody (ab121303)
ab121303 at 1/100 dilution staining COMMD9 in Paraffin-embedded Human small intestine tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"