Overview

  • Product name
  • Description
    Rabbit polyclonal to COMMD9
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 41-171 of Human COMMD9.

  • Positive control
    • COMMD9-transfected HEK293T lysate; Human small intestine tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and golgi apparatus. Recommended concentration of ab121303 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-COMMD9 antibody (ab121303) at 1/250 dilution

    Lane 1 : vector only transfected HEK293T lysate
    Lane 2 : COMMD9 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells

    Developed using the ECL technique10-20% SDS PAGE
  • ab121303 at 1/100 dilution staining COMMD9 in Paraffin-embedded Human small intestine tissue by Immunohistochemistry.

References

ab121303 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab121303.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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