• Product name
  • Description
    Rabbit polyclonal to COMP
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Cow, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human COMP.

    (Peptide available as ab74523.)

  • Positive control
    • This antibody gave a positive signal in mouse cartilage, human skeletal muscle and human heart tissue lysates.



Our Abpromise guarantee covers the use of ab74524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 82 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 21939397


  • Function
    May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7.
  • Tissue specificity
    Abundantly expressed in the chondrocyte extracellular matrix, and is also found in bone, tendon, ligament and synovium and blood vessels. Increased amounts are produced during late stages of osteoarthritis in the area adjacent to the main defect.
  • Involvement in disease
    Defects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1) [MIM:132400]. EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types.
    Defects in COMP are the cause of pseudoachondroplasia (PSACH) [MIM:177170]. PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood.
  • Sequence similarities
    Belongs to the thrombospondin family.
    Contains 4 EGF-like domains.
    Contains 1 TSP C-terminal (TSPC) domain.
    Contains 8 TSP type-3 repeats.
  • Developmental stage
    Present during the earliest stages of limb maturation and is later found in regions where the joints develop.
  • Domain
    The cell attachment motif mediates the attachment to chondrocytes. It mediates the induction of both the IAP family of survival proteins and the antiapoptotic response.
    The TSP C-terminal domain mediates interaction with FN1 and ACAN.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple) antibody
    • Cartilage oligomeric matrix protein antibody
    • Cartilage oligomeric matrix protein precursor antibody
    • COMP antibody
    • COMP_HUMAN antibody
    • EDM 1 antibody
    • EDM1 antibody
    • EPD 1 antibody
    • EPD1 antibody
    • Epiphyseal dysplasia 1 antibody
    • Epiphyseal dysplasia 1 multiple antibody
    • Epiphyseal dysplasia multiple 1 antibody
    • MED antibody
    • MGC13181 antibody
    • MGC149768 antibody
    • PSACH antibody
    • pseudoachondroplasia (epiphyseal dysplasia 1, multiple) antibody
    • Pseudoachondroplasia antibody
    • THBS 5 antibody
    • THBS5 antibody
    • Thrombospondin 5 antibody
    • Thrombospondin-5 antibody
    • Thrombospondin5 antibody
    • TSP5 antibody
    see all


  • All lanes : Anti-COMP antibody (ab74524) at 1 µg/ml

    Lane 1 : Mouse Cartilage Tissue Lysate
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 4 : Human heart tissue lysate - total protein (ab29431)
    Lane 5 : Heart (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 82 kDa
    Observed band size: 100,80 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 150 seconds

    Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.


This product has been referenced in:
  • Hakimi O  et al. A quantitative label-free analysis of the extracellular proteome of human supraspinatus tendon reveals damage to the pericellular and elastic fibre niches in torn and aged tissue. PLoS One 12:e0177656 (2017). Read more (PubMed: 28542244) »
  • Jeong JW  et al. Mori Folium water extract alleviates articular cartilage damages and inflammatory responses in monosodium iodoacetate-induced osteoarthritis rats. Mol Med Rep 16:3841-3848 (2017). Read more (PubMed: 29067461) »

See all 5 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Human cartilage and bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Human cartilage and bone
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 488µg/mL · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jan 25 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Horse Tissue sections (cartilage)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K in 0.05 M Tris-HCl, pH 7,6

Marjan Steppe

Verified customer

Submitted Dec 07 2016

Thank you for contacting us.

The antibody will most likely be okay. If you have some samples that aren't too precious, you may want to try a WB or even dot blot to see if you get reactivity. Storage at 4C is not ideal, but the antibody may n...

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Thank you for contacting Abcam.

The anti-COMP antibody, ab74524 has not been tested in immunofluoresce. If we have tested an antibody in a particular application and it failed to give any signal, then we will put that information on the datash...

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Thank you for your reply.

As ab42256 is specific peptide, there is no alternative for it. Also, with this product there is currently a 1-2 week wait until we are able to send it out. With this product, we only begin the restocking process once...

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Thank you for contacting Abcam.

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ab7973 alternative

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