Anti-COMP antibody [MA37C94 (HC484D1)] (ab11056)

Overview

  • Product nameAnti-COMP antibody [MA37C94 (HC484D1)]
    See all COMP primary antibodies
  • Description
    Rat monoclonal [MA37C94 (HC484D1)] to COMP
  • SpecificityAb11056 recognises human COMP.
  • Tested applicationsSuitable for: WB, IHC-Fr, IHC-P, ELISA, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human cartilage derived COMP

  • EpitopeThe antibody recognises an epitope located in the central portion of the molecule. Unfortunately, we do not have information regarding the exact region.
  • General notesStorage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Target

  • FunctionMay play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7.
  • Tissue specificityAbundantly expressed in the chondrocyte extracellular matrix, and is also found in bone, tendon, ligament and synovium and blood vessels. Increased amounts are produced during late stages of osteoarthritis in the area adjacent to the main defect.
  • Involvement in diseaseDefects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1) [MIM:132400]. EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types.
    Defects in COMP are the cause of pseudoachondroplasia (PSACH) [MIM:177170]. PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood.
  • Sequence similaritiesBelongs to the thrombospondin family.
    Contains 4 EGF-like domains.
    Contains 1 TSP C-terminal (TSPC) domain.
    Contains 8 TSP type-3 repeats.
  • Developmental stagePresent during the earliest stages of limb maturation and is later found in regions where the joints develop.
  • DomainThe cell attachment motif mediates the attachment to chondrocytes. It mediates the induction of both the IAP family of survival proteins and the antiapoptotic response.
    The TSP C-terminal domain mediates interaction with FN1 and ACAN.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple) antibody
    • Cartilage oligomeric matrix protein antibody
    • Cartilage oligomeric matrix protein precursor antibody
    • COMP antibody
    • COMP_HUMAN antibody
    • EDM 1 antibody
    • EDM1 antibody
    • EPD 1 antibody
    • EPD1 antibody
    • Epiphyseal dysplasia 1 antibody
    • Epiphyseal dysplasia 1 multiple antibody
    • Epiphyseal dysplasia multiple 1 antibody
    • MED antibody
    • MGC13181 antibody
    • MGC149768 antibody
    • PSACH antibody
    • pseudoachondroplasia (epiphyseal dysplasia 1, multiple) antibody
    • Pseudoachondroplasia antibody
    • THBS 5 antibody
    • THBS5 antibody
    • Thrombospondin 5 antibody
    • Thrombospondin-5 antibody
    • Thrombospondin5 antibody
    • TSP5 antibody
    see all

References for Anti-COMP antibody [MA37C94 (HC484D1)] (ab11056)

This product has been referenced in:
  • Posey KL  et al. Antioxidant and anti-inflammatory agents mitigate pathology in a mouse model of pseudoachondroplasia. Hum Mol Genet 24:3918-28 (2015). IHC ; Mouse . Read more (PubMed: 25859006) »
  • Posey KL  et al. Chondrocyte-specific pathology during skeletal growth and therapeutics in a murine model of pseudoachondroplasia. J Bone Miner Res 29:1258-68 (2014). IHC ; Mouse . Read more (PubMed: 24194321) »

See all 5 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0, microwave
Sample Human Tissue sections (human cartilage)
Specification human cartilage
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
Username

Dr. Steffen Rickelt

Verified customer

Submitted Mar 19 2014

DISCOUNT CODE: 100%0000

Ablaufdatum: 22. Oktober 2012

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I"m sorry this vial of ab11056 is giving Xiangyuan trouble. I am happy to provide a refund if this antibody has been purchased within the last 6 months or so. I will just need a PO number or Abcam order reference n...

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Thank you for your enquiry. The antibody ab11056 is a tissue culture supernatant, therefore we can't give a precise concentration. Supernatants will typically be between 10 and 50 ug/ml specific antibody. IHC staining is described in: Di Cesare ...

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