Complement C3a des Arg Human ELISA Kit (ab133037)

Overview

  • Product nameComplement C3a des Arg Human ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    High conc. 16 12.3ng/ml 11.1%
    Low conc. 16 1.86ng/ml 8.7%
    Medium conc. 16 3.4ng/ml 9.8%
  • Sample type
    Plasma
  • Assay typeCompetitive
  • Sensitivity
    0.12 ng/ml
  • Range
    0.313 ng/ml - 20 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Plasma 94.8 % - %

  • Assay time
    3h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Complement C3a des Arg Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is for the accurate quantitation of Human Complement C3a des Arg in plasma.

    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Complement C3a des Arg antigen and a polyclonal rabbit antibody specific to Complement C3a des Arg. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Complement C3a des Arg captured in the plate.

  • Notes

    The Human C3a des Arg molecule is one of three activation fragments formed from the activation of the complement cascade. C3a des Arg is formed from C3a via carboxypeptidase cleavage of the C-terminal arginine group. The structure of Human C3a des Arg was first reported in 1975 by Hugli. Human C3a des Arg contains 77 amino acids with 6 cysteines involved in disulfide bridges between residues 22-49, 23-56 and 36-57. The C terminal end of C3a des Arg in Human, porcine, rat, mouse and guinea pig is identical. C3a des Arg is a highly cationic molecule containing no carbohydrate. X-ray crystal data shows the N and C terminal residues to have highly flexible helical structures. C3a is one of the most potent constrictors of smooth muscle cells, and guinea pig airways are hyperresponsive to C3a when pretreated with histamine. The long term study of liver and other transplant recipients for both C3a des Arg and C4a des Arg may be useful in assessing a number of pathological conditions. The use of potent protease inhibitors, such as Futhan, in conjunction with EDTA, may allow complement activation factors to be quantitated specifically via inhibition of non-specific protease formation of C3a des Arg.

    Cross Reactivity

    CompoundCross Reactivity

    Human Complement C3a des Arg

    100%

    Human Complement C3

    1.28%

    Human Complement C4a des Arg

    0.40%
    Human Complement C4 0.31%

    Human Complement C5 des Arg

     0.10%
    Human Complement C5 0.05%
  • Tested applicationsCompetitive ELISA more details
  • PlatformMicroplate

Properties

  • RelevanceC3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • Cellular localizationSecreted
  • Alternative names
    • C3
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
    • Complement C3
    • CPAMD1
  • Database links

Applications

Our Abpromise guarantee covers the use of ab133037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Competitive ELISA Use at an assay dependent concentration.

Complement C3a des Arg Human ELISA Kit images

  • Representative Standard Curve using ab133037

Protocols

References for Complement C3a des Arg Human ELISA Kit (ab133037)

ab133037 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for your interest our products.

I am sorry to confirm that we cannot recommend this kit for rat samples at the moment without concerns. I agree that the rat and human C3a des Arg are very alike, but this kit was not tested...

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Thank you for your email.
We recommend that plasma samples should be collected in EDTA/Futhan tubes in order to avoid possible low-level complement activation.
If EDTA/Futhan tubes are not an option then EDTA could be used although EDTA/Futhan...

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Te confirmo también que este kit no se ha testado específicamente con muestras de ratón, pero esta diseñado específicamente para reconocer la proteína humana.

Espero haber resuelto todas las dudas, y ...

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Siento no haber contestado antes, me temo que no tengo la información de todos los productos solicitados, sin embargo, puedo proporcionarte acerca de los siguientes kits:

El kit PLTP Activity Fluorometric Assay Kit (ab65386) no es apro...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"