Overview

  • Product nameComplement C4 Human ELISA KitSee all Complement C4 kits ...
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.6%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.08 ng/ml
  • Range
    0.078 ng/ml - 20 ng/ml
  • Recovery

    98.5 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Complement C4 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Complement C4 levels in urine, milk, saliva and cell culture supernatants.

    A Complement C4 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C4 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C4 captured in plate.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • Alternative names
    • Acidic complement C4
    • Basic complement C4
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 2
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 3
    • C4B
    • CO4
    • Complement C4 A
    • Complement C4 B
    • Complement component 4A
    • Complement component 4B
    • CPAMD2
    • CPAMD3
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

Complement C4 Human ELISA Kit images

  • Representative Standard Curve using ab108825

Protocols

References for Complement C4 Human ELISA Kit (ab108825)

ab108825 has not yet been referenced specifically in any publications.

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