Overview

  • Product name
    Complex II WB Antibody Cocktail
    See all Complex II kits
  • Assay type
    Quantitative
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Product overview

    Contains a total of 305 µg of antibodies and is a pre-mixed solution of 3 monoclonal antibodies against Complex II subunits 70 kDa Fp, 30 kDa Ip along with an antibody against Complex V alpha as a control. This cocktail can be used to verify isolated Complex II purity or to conveniently determine the levels of 2 of the 4 Complex II subunits in cell lines relative to the control protein (Complex V alpha).

    Cocktail Antibodies:

    Mouse C-V-alpha [15H4C4] monoclonal:

    Amount: 50 µg

    Working concentration: 1 µg/ml

    Mouse C-II-30 (FeS) [21A11AE7] monoclonal:

    Amount: 250 µg

    Working concentration: 5 µg/ml

    Mouse C-II-70 (FL) [2E3GC12FB2AE2] monoclonal:

    Amount: 5 µg

    Working concentration: 0.1 µg/ml

  • Tested applications
    Suitable for: WBmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab110410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 6 µg/ml. The antibody cocktail (1.5 mg/mL) should be diluted 250x to a final working concentration of 6 µg/mL for Western blotting.

Images

  • All lanes : Complex II WB Antibody Cocktail (ab110410) at 1/250 dilution

    Lane 1 : human heart mitochondria at 10 µg
    Lane 2 : Immunoprecipitated Complex II at 0.5 µg


    The Western blot cocktail ab110410 contains two Complex II subunits SDHA (70 kDa) and SDHB (30 kDa)and a general mitochondrial marker ATP synthase alpha subunit (F1α, 55 kDa). All three are present in the mitochondrial sample (1). The Complex II sample purified by Complex II immunoprecipitation beads (ab109799) shows only Complex II subunits (2).

References

This product has been referenced in:
  • Yan H  et al. NR4A1-induced increase in the sensitivity of a human gastric cancer line to TNFa-mediated apoptosis is associated with the inhibition of JNK/Parkin-dependent mitophagy. Int J Oncol 52:367-378 (2018). Read more (PubMed: 29207128) »
  • Guerra F  et al. Placing mitochondrial DNA mutations within the progression model of type I endometrial carcinoma. Hum Mol Genet 20:2394-405 (2011). WB ; Human . Read more (PubMed: 21470976) »

See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab110410.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up