Anti-Complex III Immunocapture antibody [11A51H12] (ab109862)

Overview

  • Product nameAnti-Complex III Immunocapture antibody [11A51H12]
  • Description
    Mouse monoclonal [11A51H12] to Complex III Immunocapture
  • Specificity100 µg ab109862 can capture at least 10 µg Complex III from 1 mg solubilized bovine heart mitochondria.
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Bovine Heart Complex III

  • Positive control
    • Bovine heart and Human mitochondria
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab109862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. PubMed: 22523357
IP Use at 10-1 µg/mg of lysate.

Target

  • RelevanceComplex III, or ubiquinol cytochrome c oxidoreductase, is a complex of 11 subunits and catalyses electron transfer from reduced ubiquinone or coenzyme Q 10 to cytochrome c. The complex contains 11 different subunits, one of which, cytochrome b, is encoded on mitochondrial DNA, while the remainder subunits are nuclear-encoded. Genetic complex III deficiencies occur but are rare.

Anti-Complex III Immunocapture antibody [11A51H12] images

  • Staining of Complex III Lane 1: 0.1mg of Bovine mitochondria Lane 2: 0.05mg Bovine mitochondria Lane 3: 0.05mg Human mitochondria Immunoprecipitated using ab109862 at 10 µg/mg lysate.

References for Anti-Complex III Immunocapture antibody [11A51H12] (ab109862)

This product has been referenced in:
  • Beutner G  et al. Initiation of electron transport chain activity in the embryonic heart coincides with the activation of mitochondrial complex 1 and the formation of supercomplexes. PLoS One 9:e113330 (2014). IP ; Mouse . Read more (PubMed: 25427064) »
  • Jonckheere AI  et al. A complex V ATP5A1 defect causes fatal neonatal mitochondrial encephalopathy. Brain 136:1544-54 (2013). Read more (PubMed: 23599390) »

See all 5 Publications for this product

Product Wall

Thank you for contacting us. Please click these links to find the suitable antibodies. Type the MS code in Abcam search box to get the Abcam catalogue number. Complex I http://www.mitosciences.com/complex_i.html Complex IV http://www.mi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"