Complex IV Human Protein Quantity Dipstick Assay Kit (ab109877)

Overview

  • Product nameComplex IV Human Protein Quantity Dipstick Assay Kit
    See all Complex IV kits
  • Sample type
    Cell culture extracts, Tissue
  • Assay typeSandwich (quantitative)
  • Species reactivity
    Reacts with: Cow, Human
  • Product overview

    ab109877(MS431) contains 30 or 90 dipsticks and necessary components to quantify the levels of the fully assembled cytochrome c oxidase enzyme complex from human and bovine samples. The kit includes sufficient materials to generate a standard curve and evaluate several unknown samples.


    Based on the immunologic sandwich assay, this kit utilizes two monoclonal antibodies (mAbs) specific to different antigens present on the intact COX enzyme complex. One antibody is immobilized on nitrocellulose membrane in a thin line perpendicular to the length of the dipstick while the other is gold-conjugated for a visual signal. When assembled COX is present in the sample, a red line appears on the dipstick at the site of the COX antibody line. The signal intensity is directly related to the level of COX enzyme complex in the sample. The signal intensity is best measured by a dipstick reader or may be analyzed by another imaging system.

  • Notes

    All components are stable in their provided containers at room temperature out of direct sunlight.

    After diluting the 10X Blocking Buffer to 2X, store at 4°C.

    For long-term storage, all buffers can be stored at 4°C.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformReagents

Properties

Applications

Our Abpromise guarantee covers the use of ab109877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Complex IV Human Protein Quantity Dipstick Assay Kit images

  • An example using ab109877 to quantify Complex IV levels using various concentration of human fibroblast extract. Shown is a 1:2 dilution series using a positive control sample. Approximately 7 to 8 dipsticks are suitable for covering the entire working range and the blank for background levels. In this example the dilution series starts with 10 µg of fibroblast extract. A one-site hyperbola line was generated for best-fit analysis using GraphPad.
  • An example using ab109877 to quantify Complex IV levels using various concentration of human fibroblast extract. Based on the above standard curve values, 5 µg of protein extract were loaded to each dipstick for the four unknown samples (1-4). Using GraphPad software, the signal intensity from the standard curve was interpolated and the quantity of Complex I in samples 1-5 was determined. Based on the above analysis, the unknown samples had between 4% and 64% of Complex IV levels compared to the control.
  • Dipstick assays use the well-established lateral flow concept, whereby capture antibodies are striped onto nitrocellulose membrane and a Whatman paper wicking pad draws the sample through the antibody bands. Detector antibodies, conjugated to gold, are dried in the wells of a 96-well plate. Sample is added to the well, the dipstick inserted, and within minutes the line for each target is revealed as the protein-detector antibody-gold complex binds with the capture antibodies. Multiplexing dipstick assays have multiple target protein lines. A positive control goat anti-mouse antibody line is included on all assays to ensure that adequate wicking of the sample occurred.

Protocols

References for Complex IV Human Protein Quantity Dipstick Assay Kit (ab109877)

This product has been referenced in:
  • Sahdeo S  et al. Dyclonine rescues frataxin deficiency in animal models and buccal cells of patients with Friedreich's ataxia. Hum Mol Genet N/A:N/A (2014). Read more (PubMed: 25113747) »
  • Nadanaciva S  et al. Lateral-flow immunoassay for detecting drug-induced inhibition of mitochondrial DNA replication and mtDNA-encoded protein synthesis. J Immunol Methods 343:1-12 (2009). Read more (PubMed: 19152798) »

See all 4 Publications for this product

Product Wall

Unfortunately ab109877 will not work with rat samples.
We do have an activity assay for rodent in dipstick format that I think may serve the same purpose:
http://www.abcam.com/complex-iv-rodent-enzyme-activity-dipstick-assay-kit-ab109878.html

Thank you for contacting us.

We don't have exactly what you're looking for unfortunately, but we do have MetaPath™ Mito Disease 4-Plex Dipstick Array, ab109879.

The ab109879 is a novel array that allows for the simultaneous ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"