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ab109877(MS431) contains 30 or 90 dipsticks and necessary components to quantify the levels of the fully assembled cytochrome c oxidase enzyme complex from human and bovine samples. The kit includes sufficient materials to generate a standard curve and evaluate several unknown samples.
Based on the immunologic sandwich assay, this kit utilizes two monoclonal antibodies (mAbs) specific to different antigens present on the intact COX enzyme complex. One antibody is immobilized on nitrocellulose membrane in a thin line perpendicular to the length of the dipstick while the other is gold-conjugated for a visual signal. When assembled COX is present in the sample, a red line appears on the dipstick at the site of the COX antibody line. The signal intensity is directly related to the level of COX enzyme complex in the sample. The signal intensity is best measured by a dipstick reader or may be analyzed by another imaging system.
All components are stable in their provided containers at room temperature out of direct sunlight.
After diluting the 10X Blocking Buffer to 2X, store at 4°C.
For long-term storage, all buffers can be stored at 4°C.
|Buffer B (10X Blocking solution)||1 x 0.4ml|
|Dipsticks||1 x 48 units|
|Extraction Buffer||1 x 15ml|
|Gold-conjugated antibody (dried in microplate wells)||1 x 48 tests|
|Wash buffer||1 x 2ml|
Our Abpromise guarantee covers the use of ab109877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|