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ab109878(MS432) contains 30 or 90 dipsticks and necessary components to quantify the activity of the cytochrome c oxidase enzyme complex from mouse and rat samples. The kit includes sufficient materials to generate a standard curve and evaluate several unknown samples.
Using an antibody immobilized on the dipstick that specifically recognizes COX, the enzyme complex is immno-captured (i.e immuno-precipitated in active form) and enzyme activity is determined directly on the dipstick. Using a traditional method for determining COX enzyme activity by histo-chemical methods and in-gel activity assays, Di-amino benzidinetetrachloride (DAB) serves as the reporter of COX activity. The greater the signal of precipitated DAB, the greater the amount of COX enzyme complex. In addition, the reaction is cyanide sensitive. The signal intensity is best measured by a dipstick reader or may be analyzed by another imaging system.
Store dipsticks at room temperature in their provided container and out of direct sunlight.
High humidity conditions should be avoided.
Store Buffer A, B, and C at 4°C or at -20°C for long term storage.
Store Tubes 1 and 2 at -20°C or at -80°C for long term storage; they can also be aliquoted upon receipt to prevent freeze/thaw cycles.
Tube 3 can be stored at room temperature.
|Components||90 tests||30 tests|
|10X Blocking Buffer||3 x 2ml||1 x 2ml|
|96-well microplate||6 units||2 units|
|Dipsticks||1 x 90 units||1 x 30 units|
|Extraction Buffer||3 x 15ml||1 x 15ml|
|Tube 1 (DAB 100X stock)||3 x 100µl||1 x 100µl|
|Tube 2 (reduced cytochrome c 20X stock)||3 x 500µl||1 x 500µl|
|Tube 3 (Buffer for activity assay)||3 x 10ml||1 x 10ml|
|Wash buffer||3 x 1ml||1 x 1ml|
Our Abpromise guarantee covers the use of ab109878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|