The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 - 1 µg/ml. Approx 26kDa plus 29kDa bands observed in Human Testis lysates (calculated MW of 30.0kDa according to NP_000745.1 and of 24.5kDa according to NP_009294.1).
FunctionCatalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
Tissue specificityBrain, liver, placenta, lymphocytes and erythrocytes.
Sequence similaritiesBelongs to the mammalian catechol-O-methyltransferase family.
Post-translational modificationsThe N-terminus is blocked.
Glen A et al. iTRAQ-Facilitated Proteomic Analysis of Human Prostate Cancer Cells Identifies Proteins Associated with Progression. J Proteome Res7:897-907 (2008).
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