Overview

  • Product name
  • Description
    Goat polyclonal to COMT
  • Specificity
    This antibody is expected to recognise both reported isoforms (NP_000745.1 and NP_009294.1).
  • Tested applications
    Suitable for: WB, IHC-P, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Dog, Pig
  • Immunogen

    Synthetic peptide:

    C-QDIIPQLKKKYDVD

    , corresponding to internal sequence amino acids 170-183 of Human COMT

  • Positive control
    • Human Testis lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab51984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects an approximate 28kDa band and faint 25kDa band observed in human testis lysates (calculated MW of 30.0kDa according to NP_000745.1 and 24.5kDa according to NP_009294.1).
IHC-P Use a concentration of 2 - 4 µg/ml.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Increasing amount of recombinant COMTcaptured by a rabbit antibody. ab51984 used as reporter.

Target

Images

  • Immunohistochemistry (paraffin-embedded sections) analysis of human cerebellum tissue labeling COMT with ab51984 at 4µg/ml. Microwaved antigen retrieval was performed using Tris/EDTA buffer pH 9. HRP staining.

  • Anti-COMT antibody (ab51984) at 0.03 µg/ml + Human Testis lysate in RIPA buffer at 35 µg

    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour. Detected by chemiluminescence.

References

ab51984 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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