The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.03 - 0.1 µg/ml. Detects an approximate 28kDa band and faint 25kDa band observed in human testis lysates (calculated MW of 30.0kDa according to NP_000745.1 and 24.5kDa according to NP_009294.1).
Use a concentration of 2 - 4 µg/ml.
Use a concentration of 0.5 - 2 µg/ml. Increasing amount of recombinant COMTcaptured by a rabbit antibody. ab51984 used as reporter.
FunctionCatalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
Tissue specificityBrain, liver, placenta, lymphocytes and erythrocytes.
Sequence similaritiesBelongs to the mammalian catechol-O-methyltransferase family.
Post-translational modificationsThe N-terminus is blocked.
Immunohistochemistry (paraffin-embedded sections) analysis of human cerebellum tissue labeling COMT with ab51984 at 4µg/ml. Microwaved antigen retrieval was performed using Tris/EDTA buffer pH 9. HRP staining.
Western blot - COMT antibody (ab51984)
Anti-COMT antibody (ab51984) at 0.03 µg/ml + Human Testis lysate in RIPA buffer at 35 µg