Validated using a knockout cell line
Recombinant
RabMAb

Anti-COMT antibody [EPR6490] (ab126618)

Overview

  • Product name
    Anti-COMT antibody [EPR6490]
    See all COMT primary antibodies
  • Description
    Rabbit monoclonal [EPR6490] to COMT
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human COMT. (Uniprot: P21964).

  • Positive control
    • Human fetal liver, A431, MOLT4 and 293T lysates' Human kidney and liver tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab126618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 24-30 kDa (predicted molecular weight: 30 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    Images



    • Predicted band size : 30 kDa

      Lane 1: Wild type HAP1 whole cell lysate (40 µg)
      Lane 2: COMT knockout  HAP1 whole cell lysate (40 µg)
      Lane 3: A431 whole cell lysate (20 µg)
      Lane 4: MCF7 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab126618 observed at 24/28 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab126618 was shown to specifically react with COMT when COMT knockout samples were used. Wild-type and COMT knockout samples were subjected to SDS-PAGE. Samples were incubated with ab126618 and ab8245 (Mouse anti-GAPDH loading control) overnight at 4°C at 1/1000 and 1/10000 dilutions respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • ab126618, at 1/50 dilution, staining COMT in Formalin-fixed, Paraffin-embedded Human liver tissue by Immunohistochemistry.
    • All lanes : Anti-COMT antibody [EPR6490] (ab126618) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : A431 cell lysate
      Lane 3 : MOLT4 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 30 kDa
    • ab126618, at 1/50 dilution, staining COMT in Formalin-fixed, Paraffin-embedded Human kidney tissue by Immunohistochemistry.

    References

    This product has been referenced in:
    • Stefanovic B  et al. Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels. Eur Neuropsychopharmacol 26:1629-37 (2016). WB ; Rat . Read more (PubMed: 27499503) »
    • Wakuda T  et al. Perinatal asphyxia alters neuregulin-1 and COMT gene expression in the medial prefrontal cortex in rats. Prog Neuropsychopharmacol Biol Psychiatry 56:149-54 (2015). Read more (PubMed: 25194460) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Liver)
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 25 2016

    Tal y como supones, el anticuerpo detecta únicamente la isoforma unida a la membrana.

    Si tienes cualquier otra consulta, no dudes en contactarme de nuevo. Recuerda que todos nuestros productos tienen 6 meses de garantía, y si no...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up