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Synthetic peptide, corresponding to residues in Human COMT. (Uniprot: P21964).
This product is a recombinant rabbit monoclonal antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab126618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 24-30 kDa (predicted molecular weight: 30 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Lane 1: Wild type HAP1 whole cell lysate (40 µg)
Lane 2: COMT knockout HAP1 whole cell lysate (40 µg)
Lane 3: A431 whole cell lysate (20 µg)
Lane 4: MCF7 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab126618 observed at 24/28 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab126618 was shown to specifically react with COMT when COMT knockout samples were used. Wild-type and COMT knockout samples were subjected to SDS-PAGE. Samples were incubated with ab126618 and ab8245 (Mouse anti-GAPDH loading control) overnight at 4°C at 1/1000 and 1/10000 dilutions respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"