Overview

  • Product nameAnti-COMT antibody [EPR6491(B)]
    See all COMT primary antibodies
  • Description
    Rabbit monoclonal [EPR6491(B)] to COMT
  • Tested applicationsSuitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human COMT.

  • Positive control
    • Fetal Liver, A431 and MOLT4 cell lysates; A431 cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab124813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 24-30 kDa (predicted molecular weight: 30 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC,IHC-P or IP.
  • Target

    • FunctionCatalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
    • Tissue specificityBrain, liver, placenta, lymphocytes and erythrocytes.
    • Sequence similaritiesBelongs to the mammalian catechol-O-methyltransferase family.
    • Post-translational
      modifications
      The N-terminus is blocked.
    • Cellular localizationCytoplasm and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Catechol O methyltransferase antibody
      • Catechol O-methyltransferase antibody
      • COMT antibody
      • COMT_HUMAN antibody
      • EC 2.1.1.6 antibody
      see all

    Anti-COMT antibody [EPR6491(B)] images



    • Predicted band size : 30 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: COMT knockout HAP1 whole cell lysate (20 µg)
      Lane 3: A431 whole cell lysate (20 µg)
      Lane 4: MOLT4 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab124813 observed at 28 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab124813 was shown to recognize COMT when COMT knockout samples were used, along with additional cross-reactive bands. Wild-type and COMT knockout samples were subjected to SDS-PAGE.  Ab124813 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-COMT antibody [EPR6491(B)] (ab124813) at 1/1000 dilution

      Lane 1 : Fetal Liver lysate
      Lane 2 : A431 cell lysate
      Lane 3 : MOLT4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique

      Predicted band size : 30 kDa
    • ab124813, at 1/10 dilution staining COMT in A431 cells, (Red) compared to a nonspecific negative control antibody (Green) by Flow Cytometry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-COMT antibody [EPR6491(B)] (ab124813)

    ab124813 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab124813.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"