Validated using a knockout cell line
Recombinant
RabMAb

Anti-COMT antibody [EPR6491(B)] (ab124813)

Overview

  • Product name
    Anti-COMT antibody [EPR6491(B)]
    See all COMT primary antibodies
  • Description
    Rabbit monoclonal [EPR6491(B)] to COMT
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human COMT.

  • Positive control
    • Human fetal Liver, A431 and MOLT4 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 24-30 kDa (predicted molecular weight: 30 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
    • Tissue specificity
      Brain, liver, placenta, lymphocytes and erythrocytes.
    • Sequence similarities
      Belongs to the mammalian catechol-O-methyltransferase family.
    • Post-translational
      modifications
      The N-terminus is blocked.
    • Cellular localization
      Cytoplasm and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Catechol O methyltransferase antibody
      • Catechol O-methyltransferase antibody
      • COMT antibody
      • COMT_HUMAN antibody
      • EC 2.1.1.6 antibody
      see all

    Images



    • Predicted band size : 30 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: COMT knockout HAP1 whole cell lysate (20 µg)
      Lane 3: A431 whole cell lysate (20 µg)
      Lane 4: MOLT4 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab124813 observed at 28 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab124813 was shown to recognize COMT when COMT knockout samples were used, along with additional cross-reactive bands. Wild-type and COMT knockout samples were subjected to SDS-PAGE.  Ab124813 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-COMT antibody [EPR6491(B)] (ab124813) at 1/1000 dilution

      Lane 1 : Human fetal Liver lysate
      Lane 2 : A431 cell lysate
      Lane 3 : MOLT4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique

      Predicted band size : 30 kDa
    • ab124813, at 1/10 dilution staining COMT in A431 cells, (Red) compared to a nonspecific negative control antibody (Green) by Flow Cytometry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab124813 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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