Anti-conjugated Octopamine antibody (ab37092)

Overview

  • Product nameAnti-conjugated Octopamine antibody
  • Description
    Rabbit polyclonal to conjugated Octopamine
  • SpecificityThis antibody targets conjugated octopamine. This antibody does not recognize free octopamine. Using a conjugate octopamine-protein carrier (Pc), antibody specificity was performed with an ELISA test by competition experiments with the following compounds: cross reactivity§ Octopamine-G-(Pc):1 Tyramine-G-(Pc):1/400 Naradrenaline-G-(Pc):1/10,000 Tyrosine-G-(Pc):1/>50,000 Dopamine-G-(Pc):1/100,000 G = Glutaraldehyde § Octopamine-G-(Pc) concentration/other conjugated catecholamine concentration at half displacement.
  • Tested applicationsSuitable for: IHC-Glut, ELISA, ICC, WBmore details
  • Species reactivity
    not applicable
  • Immunogen

    Chemical/ Small Molecule corresponding to conjugated Octopamine.

Properties

Applications

Our Abpromise guarantee covers the use of ab37092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Glut 1/1000 - 1/5000. Fixation should be done with gluatraldehyde. The use of PFA with glutaraldehyde may improve staining results.
ELISA 1/1000 - 1/5000.
ICC 1/1000 - 1/5000. Fixation should be done with gluatraldehyde. The use of PFA with glutaraldehyde may improve staining results.
WB 1/1000 - 1/2000. Predicted molecular weight: 1 kDa.

This antibody does not recognize free octopamine.

References for Anti-conjugated Octopamine antibody (ab37092)

This product has been referenced in:
  • Châtel A  et al. Characterization of tyramine ß-hydroxylase, an enzyme upregulated by stress in Periplaneta americana. J Mol Endocrinol 50:91-102 (2013). ELISA . Read more (PubMed: 23197360) »

See 1 Publication for this product

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Unfortunately the supplying laboratory didn't have the image available for this product but we would still guarantee the product for use in WB.

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Thank you for contacting us with your question. Our datasheet did say that formaldehyde is not a suitable fixative to use with this antibody, however I could not find any data regarding this statement. I have reviewed the notes from the testing ...

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Application Immunohistochemistry (PFA fixed)
Sample Fruit fly (Drosophila melanogaster) Cell (drosophila neuromuscular junction)
Specification drosophila neuromuscular junction
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Submitted Feb 26 2010

Application Immunocytochemistry/ Immunofluorescence
Sample Fruit fly (Drosophila melanogaster) Cultured Cells (cultured neurons)
Specification cultured neurons
Fixative Paraformaldehyde
Permeabilization Yes - PBST
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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Submitted Feb 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"