Anti-Connexin 30.3 / GJB4 antibody (ab32598)

Overview

  • Product nameAnti-Connexin 30.3 / GJB4 antibody
    See all Connexin 30.3 / GJB4 primary antibodies
  • Description
    Rabbit polyclonal to Connexin 30.3 / GJB4
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: GPNAPSLYDN LSKKRGGLWW TYLLSLIFKA AVDAGFLYIF HRLYKDYDMP, corresponding to internal sequence amino acids 110-159 of Human Connexin 30.3/ GJB4

  • Positive control
    • Jurkat cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/1.5625e+006.

Target

  • RelevanceConnexins are homologous four transmembrane domain proteins and major components of gap junctions. The GJB4 gene encodes connexin 30.3 (Cx30.3). A mutation in connexin 30.3 is causally involved in erythrokeratodermia variabilis (EKV), a mostly autosomal dominant disorder of keratinization.
  • Cellular localizationPlasma membrane
  • Database links
  • Alternative names
    • Connexin 30.3 antibody
    • Cx30.3 antibody
    • Gap junction beta 4 protein antibody

Anti-Connexin 30.3 / GJB4 antibody images

  • Anti-Connexin 30.3 / GJB4 antibody (ab32598) at 1.25 µg/ml + Jurkat cell lysate at 1 mg/ml

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Connexin 30.3 / GJB4 antibody (ab32598)

ab32598 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"