Overview

  • Product name
    Anti-Connexin 32 / GJB1 antibody
    See all Connexin 32 / GJB1 primary antibodies
  • Description
    Rabbit polyclonal to Connexin 32 / GJB1
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: CARRAQRRSN PPSRKGSGFG HRLSPEYKQN EINKLLSEQD GSLKDILRRS, corresponding to C terminal amino acids 217-266 of Human Connexin 32/ GJB1

  • Positive control
    • Jurkat cell lysate, human intestinal tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23973667
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function
    One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
  • Involvement in disease
    Defects in GJB1 are the cause of Charcot-Marie-Tooth disease X-linked type 1 (CMTX1) [MIM:302800]; also designated CMT-X. CMTX1 is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies characterized by severely reduced motor nerve conduction velocities (NCVs) (less than 38m/s) and segmental demyelination and remyelination, and primary peripheral axonal neuropathies characterized by normal or mildly reduced NCVs and chronic axonal degeneration and regeneration on nerve biopsy. CMTX1 has both demyelinating and axonal features. Central nervous system involvement may occur.
    Defects in GJB1 may contribute to the phenotype of Dejerine-Sottas syndrome (DSS) [MIM:145900]; also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS is a severe degenerating neuropathy of the demyelinating Charcot-Marie-Tooth disease category, with onset by age 2 years. DSS is characterized by motor and sensory neuropathy with very slow nerve conduction velocities, increased cerebrospinal fluid protein concentrations, hypertrophic nerve changes, delayed age of walking as well as areflexia. There are both autosomal dominant and autosomal recessive forms of Dejerine-Sottas syndrome.
  • Sequence similarities
    Belongs to the connexin family. Beta-type (group I) subfamily.
  • Cellular localization
    Cell membrane. Cell junction > gap junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • Charcot Marie Tooth neuropathy X linked antibody
    • CMTX 1 antibody
    • CMTX antibody
    • CMTX1 antibody
    • Connexin-32 antibody
    • Connexin32 antibody
    • CX 32 antibody
    • Cx32 antibody
    • CXB1_HUMAN antibody
    • GAP junction 28 kDa liver protein antibody
    • Gap junction beta 1 protein antibody
    • Gap junction beta-1 protein antibody
    • Gap junction protein beta 1 32kD antibody
    • Gap junction protein beta 1 antibody
    • GJB 1 antibody
    • GJB1 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of rat neuron cells co-cultured with rat MSCs labelling Connexin 32 / GLB1 wiith ab66613 at 1/50. Cultures were fixed with 4% paraformaldehyde. Green - Cx32, Red - Phalloidin, Blue - MAP2, Propidium Iodide - nuclei. Scale Bar 25 µm.

    After 30 days of culture, Connexin 32 was present only in co-cultured neurons but absent in neurons cultured alone and in MSCs.

    Confocal analysis of Z axis revealed that Connexin 32 was adherent to the axons.

  • Anti-Connexin 32 / GJB1 antibody (ab66613) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling Connexin 32 / GJB1 with ab66613 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the intestinal villus. Magnification: 400X.

References

This product has been referenced in:
  • Kim S  et al. The basic helix-loop-helix transcription factor E47 reprograms human pancreatic cancer cells to a quiescent acinar state with reduced tumorigenic potential. Pancreas 44:718-27 (2015). ICC/IF . Read more (PubMed: 25894862) »
  • Ravasi M  et al. Undifferentiated MSCs are able to myelinate DRG neuron processes through p75. Exp Cell Res 319:2989-99 (2013). ICC/IF ; Rat . Read more (PubMed: 23973667) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
(agent) for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample
Cow Tissue sections (Fetal Liver)
Specification
Fetal Liver
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Juliana Shimara Pires Ferrão

Verified customer

Submitted Nov 26 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (aglandular stomach, epithelium)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
aglandular stomach, epithelium
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Methacarn
Username

F Hernandez-Blazquez

Verified customer

Submitted Mar 16 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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