Overview

  • Product name
    Anti-Connexin 36 / GJA9 antibody
    See all Connexin 36 / GJA9 primary antibodies
  • Description
    Rabbit polyclonal to Connexin 36 / GJA9
  • Specificity
    This antibody is specific for Connexin 36 / GJA9
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog, Chimpanzee
  • Immunogen

    A region within synthetic peptide: QNAIVNGVLQ NTENTSKETE PDCLEVKELT PHPSGLRTAS KSKLRRQEGI, corresponding to internal sequence amino acids 146-195 of Human Connexin 36/ GJA9

  • Positive control
    • Fetal skeletal muscle lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab48814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/312500.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-Connexin 36 / GJA9 antibody (ab48814) at 1.25 µg/ml + Fetal skeletal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Farnsworth NL  et al. Low Level Pro-inflammatory Cytokines Decrease Connexin36 Gap Junction Coupling in Mouse and Human Islets through Nitric Oxide-mediated Protein Kinase Cd. J Biol Chem 291:3184-96 (2016). Read more (PubMed: 26668311) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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