• Product nameAnti-Connexin 37 / GJA4 antibody
    See all Connexin 37 / GJA4 primary antibodies
  • Description
    Rabbit polyclonal to Connexin 37 / GJA4
  • Tested applicationsSuitable for: IP, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    a region within synthetic peptide: SHIRYWVLQF LFVSTPTLIY LGHVIYLSRR EERLRQKEGE LRALPSKDLH, corresponding to N terminal amino acids 73-122 of Mouse Connexin 37/ GJA4

  • Positive control
    • NIH/3T3 membrane lysate



Our Abpromise guarantee covers the use of ab58918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay, 1:312500.

WB Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • FunctionOne gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
  • Tissue specificityExpressed in multiple organs and tissues, including heart, uterus, ovary, and blood vessel endothelium.
  • Sequence similaritiesBelongs to the connexin family. Alpha-type (group II) subfamily.
  • Cellular localizationCell membrane. Cell junction > gap junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • Connexin 37 antibody
    • Connexin-37 antibody
    • Cx37 antibody
    • CXA4_HUMAN antibody
    • Gap junction alpha 4 protein antibody
    • Gap junction alpha-4 protein antibody
    • gap junction protein, alpha 4 37kDa (connexin 37) antibody
    • gap junction protein, alpha 4, 37kD (connexin 37) antibody
    • Gap junction protein, alpha 4, 37kDa antibody
    • Gja4 antibody
    • OTTHUMP00000004181 antibody
    • OTTHUMP00000004182 antibody
    see all

Anti-Connexin 37 / GJA4 antibody images

  • Connexin 37 / GJA4 was immunoprecipitated using 0.5mg NIH3T3 whole cell extract, 5µg of Rabbit polyclonal to Connexin 37 / GJA4 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, NIH3T3 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab58918.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 45kDa; Connexin 37 / GJA4

  • Anti-Connexin 37 / GJA4 antibody (ab58918) at 10 µg/ml + NIH/3T3 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 37 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

References for Anti-Connexin 37 / GJA4 antibody (ab58918)

This product has been referenced in:
  • Yang Y  et al. The Increased Expression of Connexin and VEGF in Mouse Ovarian Tissue Vitrification by Follicle Stimulating Hormone. Biomed Res Int 2015:397264 (2015). IHC (PFA fixed) ; Mouse . Read more (PubMed: 26539488) »
  • González-Andrades M  et al. Sequential development of intercellular junctions in bioengineered human corneas. J Tissue Eng Regen Med 3:442-9 (2009). Read more (PubMed: 19415764) »

See all 2 Publications for this product

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