• Product name
    Anti-Connexin 37 / GJA4 antibody
    See all Connexin 37 / GJA4 primary antibodies
  • Description
    Rabbit polyclonal to Connexin 37 / GJA4
  • Tested applications
    Suitable for: IP, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    a region within synthetic peptide: SHIRYWVLQF LFVSTPTLIY LGHVIYLSRR EERLRQKEGE LRALPSKDLH, corresponding to N terminal amino acids 73-122 of Mouse Connexin 37/ GJA4

  • Positive control
    • NIH/3T3 membrane lysate



Our Abpromise guarantee covers the use of ab58918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay, 1:312500.

WB Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function
    One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
  • Tissue specificity
    Expressed in multiple organs and tissues, including heart, uterus, ovary, and blood vessel endothelium.
  • Sequence similarities
    Belongs to the connexin family. Alpha-type (group II) subfamily.
  • Cellular localization
    Cell membrane. Cell junction > gap junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • Connexin 37 antibody
    • Connexin-37 antibody
    • Cx37 antibody
    • CXA4_HUMAN antibody
    • Gap junction alpha 4 protein antibody
    • Gap junction alpha-4 protein antibody
    • gap junction protein, alpha 4 37kDa (connexin 37) antibody
    • gap junction protein, alpha 4, 37kD (connexin 37) antibody
    • Gap junction protein, alpha 4, 37kDa antibody
    • Gja4 antibody
    • OTTHUMP00000004181 antibody
    • OTTHUMP00000004182 antibody
    see all

Anti-Connexin 37 / GJA4 antibody images

  • Connexin 37 / GJA4 was immunoprecipitated using 0.5mg NIH3T3 whole cell extract, 5µg of Rabbit polyclonal to Connexin 37 / GJA4 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, NIH3T3 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab58918.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 45kDa; Connexin 37 / GJA4

  • Anti-Connexin 37 / GJA4 antibody (ab58918) at 10 µg/ml + NIH/3T3 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 37 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

References for Anti-Connexin 37 / GJA4 antibody (ab58918)

This product has been referenced in:
  • Yang Y  et al. The Increased Expression of Connexin and VEGF in Mouse Ovarian Tissue Vitrification by Follicle Stimulating Hormone. Biomed Res Int 2015:397264 (2015). IHC (PFA fixed) ; Mouse . Read more (PubMed: 26539488) »
  • Tsang H  et al. Role of asymmetric methylarginine and connexin 43 in the regulation of pulmonary endothelial function. Pulm Circ 3:675-91 (2013). WB . Read more (PubMed: 24618552) »

See all 3 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab58918.
Please use the links above to contact us or submit feedback about this product.


Sign up