Anti-Connexin 40 / GJA5 antibody (ab113534)

Overview

  • Product nameAnti-Connexin 40 / GJA5 antibody
    See all Connexin 40 / GJA5 primary antibodies
  • Description
    Rabbit polyclonal to Connexin 40 / GJA5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Dog, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Connexin 40/ GJA5.

  • Positive control
    • This antibody gave a positive signal in both JEG3 and A549 whole cell lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab113534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).

Target

  • FunctionOne gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
  • Involvement in diseaseDefects in GJA5 are a cause of familial atrial standstill (FAS) [MIM:108770]. Atrial standstill is an extremely rare arrhythmia, characterized by the absence of electrical and mechanical activity in the atria. Electrocardiographically, it is characterized by bradycardia, the absence of P waves, and a junctional narrow complex escape rhythm.
  • Sequence similaritiesBelongs to the connexin family. Alpha-type (group II) subfamily.
  • Cellular localizationCell membrane. Cell junction > gap junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • Connexin 40 antibody
    • Connexin-40 antibody
    • Cx40 antibody
    • CXA5_HUMAN antibody
    • Gap junction alpha 5 protein antibody
    • Gap junction alpha-5 protein antibody
    • Gap junction protein alpha 5 40kD (connexin 40) antibody
    • Gap junction protein alpha 5 antibody
    • Gja5 antibody
    • MGC11185 antibody
    see all

Anti-Connexin 40 / GJA5 antibody images

  • All lanes : Anti-Connexin 40 / GJA5 antibody (ab113534) at 1 µg/ml

    Lane 1 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
    Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 40 kDa


    Exposure time : 4 minutes

References for Anti-Connexin 40 / GJA5 antibody (ab113534)

ab113534 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Monkey Tissue sections (Intestine)
Specification Intestine
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization No
Blocking step Protein Block, Serum-Free - Dako as blocking agent for 30 minute(s) · Concentration: 0.25% · Temperature: 23°C
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Submitted Mar 18 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"