Overview

  • Product name
    Anti-Connexin 40.1 antibody
  • Description
    Rabbit polyclonal to Connexin 40.1
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: KIPDEDESEV TSSASEKLGR QPRGRPHREA AQDPRGSGSE EQPSAAPSRL, corresponding to C terminal amino acids 289-338 of Human Connexin 40.1

  • Positive control
    • HepG2 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/62500.
WB Use a concentration of 0.6 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    Part of the connexin genes, the CX40.1 gene appears to have evolved to different expression patterns and presumably to different functions compared to its orthologue in the mouse genome.
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • Connexin antibody
    • CX40.1 antibody
    • Gap junction protein antibody

Anti-Connexin 40.1 antibody images

  • Anti-Connexin 40.1 antibody (ab33132) at 0.6 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-Connexin 40.1 antibody (ab33132)

ab33132 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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