Our Abpromise guarantee covers the use of ab79010 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/50 - 1/250.|
|WB||1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).Can be blocked with Connexin 43 / GJA1 peptide (ab171366).|
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||1/50 - 1/250. Use Acetone/methanol fixed tissues (1:1, 20 minutes ice cold).|