Anti-Connexin 43 / GJA1 antibody (ab87645)
Key features and details
- Goat polyclonal to Connexin 43 / GJA1
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
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Overview
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Product name
Anti-Connexin 43 / GJA1 antibody
See all Connexin 43 / GJA1 primary antibodies -
Description
Goat polyclonal to Connexin 43 / GJA1 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Rabbit, Guinea pig, Dog, Pig, Cynomolgus monkey -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab87645 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 43 kDa.
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 43 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Function
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. -
Tissue specificity
Expressed in the heart and fetal cochlea. -
Involvement in disease
Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type 3 and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances.
Defects in GJA1 are the cause of autosomal recessive oculodentodigital dysplasia (ODDD autosomal recessive) [MIM:257850].
Defects in GJA1 may be the cause of syndactyly type 3 (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected.
Defects in GJA1 are a cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
Defects in GJA1 are a cause of Hallermann-Streiff syndrome (HSS) [MIM:234100]. HSS is a disorder characterized by a typical skull shape (brachycephaly with frontal bossing), hypotrichosis, microphthalmia, cataracts, beaked nose, micrognathia, skin atrophy, dental anomalies and proportionate short stature. Mental retardation is present in a minority of cases. -
Sequence similarities
Belongs to the connexin family. Alpha-type (group II) subfamily. -
Cellular localization
Cell membrane. Cell junction > gap junction. - Information by UniProt
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Database links
- Entrez Gene: 101867099 Cynomolgus monkey
- Entrez Gene: 403418 Dog
- Entrez Gene: 100518636 Pig
- Entrez Gene: 100008935 Rabbit
- Entrez Gene: 24392 Rat
- SwissProt: Q4R4S7 Cynomolgus monkey
- SwissProt: Q6S9C0 Dog
- SwissProt: Q29101 Pig
see all -
Alternative names
- Connexin 43 antibody
- Connexin-43 antibody
- Cx 43 antibody
see all
Images
Datasheets and documents
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Datasheet download
References (10)
ab87645 has been referenced in 10 publications.
- Zhao JJ et al. The mechanisms through which auricular vagus nerve stimulation protects against cerebral ischemia/reperfusion injury. Neural Regen Res 17:594-600 (2022). PubMed: 34380899
- Lozic M et al. Alteration of Cx37, Cx40, Cx43, Cx45, Panx1, and Renin Expression Patterns in Postnatal Kidneys of Dab1-/- (yotari) Mice. Int J Mol Sci 22:N/A (2021). PubMed: 33525532
- Juric M et al. Expression of Connexins 37, 43 and 45 in Developing Human Spinal Cord and Ganglia. Int J Mol Sci 21:N/A (2020). PubMed: 33302507
- Kosovic I et al. Connexin Signaling in the Juxtaglomerular Apparatus (JGA) of Developing, Postnatal Healthy and Nephrotic Human Kidneys. Int J Mol Sci 21:N/A (2020). PubMed: 33172216
- Kosovic I et al. Spatio-temporal patterning of different connexins in developing and postnatal human kidneys and in nephrotic syndrome of the Finnish type (CNF). Sci Rep 10:8756 (2020). PubMed: 32471989
- Leffler KE & Abdel-Rahman AA Restoration of Adiponectin-Connexin43 Signaling Mitigates Myocardial Inflammation and Dysfunction in Diabetic Female Rats. J Cardiovasc Pharmacol 75:259-267 (2020). PubMed: 31868825
- Zheng L et al. Phosphorylation of Cx43 residue Y313 by Src contributes to blocking the interaction with Drebrin and disassembling gap junctions. J Mol Cell Cardiol 126:36-49 (2019). PubMed: 30448479
- Uribe P et al. Study on site-specific expression of bone formation and resorption factors in human dental follicles. Eur J Oral Sci 126:439-448 (2018). PubMed: 30216610
- Shen Y et al. Connexin 43 SUMOylation improves gap junction functions between liver cancer stem cells and enhances their sensitivity to HSVtk/GCV. Int J Oncol 52:872-880 (2018). PubMed: 29393359
- Shibata M et al. Involvement of glial cells in the autoregulation of optic nerve head blood flow in rabbits. Invest Ophthalmol Vis Sci 53:3726-32 (2012). PubMed: 22589427