Anti-Connexin 43 / GJA1 antibody [CXN-6] (ab11369)

Overview

  • Product nameAnti-Connexin 43 / GJA1 antibody [CXN-6]
    See all Connexin 43 / GJA1 primary antibodies
  • Description
    Mouse monoclonal [CXN-6] to Connexin 43 / GJA1
  • Tested applicationsSuitable for: IHC-Fr, ELISA, WB, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Hamster, Cow, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 362-381 of Connexin 43/ GJA1

  • General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
WB 1/8000 - 1/16000. Detects a band of approximately 43 kDa. Additional weak bands may be seen.
IHC-P Use at an assay dependent dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
ICC Use at an assay dependent dilution.

Target

  • FunctionOne gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.
  • Tissue specificityExpressed in the heart and fetal cochlea.
  • Involvement in diseaseDefects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type 3 and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances.
    Defects in GJA1 are the cause of autosomal recessive oculodentodigital dysplasia (ODDD autosomal recessive) [MIM:257850].
    Defects in GJA1 may be the cause of syndactyly type 3 (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected.
    Defects in GJA1 are a cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
    Defects in GJA1 are a cause of Hallermann-Streiff syndrome (HSS) [MIM:234100]. HSS is a disorder characterized by a typical skull shape (brachycephaly with frontal bossing), hypotrichosis, microphthalmia, cataracts, beaked nose, micrognathia, skin atrophy, dental anomalies and proportionate short stature. Mental retardation is present in a minority of cases.
  • Sequence similaritiesBelongs to the connexin family. Alpha-type (group II) subfamily.
  • Cellular localizationCell membrane. Cell junction > gap junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • Connexin 43 antibody
    • Connexin-43 antibody
    • Cx 43 antibody
    • Cx43 antibody
    • CXA1_HUMAN antibody
    • DFNB38 antibody
    • Gap junction 43 kDa heart protein antibody
    • Gap junction alpha-1 protein antibody
    • Gap junction protein alpha 1 43kDa (connexin 43) antibody
    • Gap junction protein alpha 1 43kDa antibody
    • Gap junction protein alpha like antibody
    • GJA 1 antibody
    • Gja1 antibody
    • GJAL antibody
    • ODD antibody
    • ODDD antibody
    • ODOD antibody
    • SDTY3 antibody
    see all

Anti-Connexin 43 / GJA1 antibody [CXN-6] images

  • Immunocytochemistry/Immunofluorescence analysis of normoxic (A) and hypoxic (B) human mesenchymal stem cells labelling Connexin 43 / GJA1 with ab11369. Blue = DAPI (scale bars = 50 μm). Cells were grown on glass coverslips in 12-well tissue-culture dishes. Coverslips were washed with DPBS and fixed with 4% paraformaldehyde for 15 min, blocked, and incubated with the primary antibody for 1 hour at 37°C. Cells were washed and incubated with FITC-conjugated secondary antibody for 1 hour at 37°C.

References for Anti-Connexin 43 / GJA1 antibody [CXN-6] (ab11369)

This product has been referenced in:
  • Wang Y  et al. The transplantation of Akt-overexpressing amniotic fluid-derived mesenchymal stem cells protects the heart against ischemia-reperfusion injury in rabbits. Mol Med Rep 14:234-42 (2016). WB ; Rabbit . Read more (PubMed: 27151366) »
  • Hou S  et al. Neuroprotective Effect of Salvianolic Acids against Cerebral Ischemia/Reperfusion Injury. Int J Mol Sci 17:N/A (2016). WB ; Rat . Read more (PubMed: 27455249) »

See all 7 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Horse Tissue sections (aglandular stomach, epithelium)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Specification aglandular stomach, epithelium
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Methacarn
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Dr. F Hernandez-Blazquez

Verified customer

Submitted Mar 16 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Horse Tissue sections (Lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH 6
Permeabilization No
Specification Lung
Blocking step BSA as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 37°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 08 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (heart, ventricular tissue)
Loading amount 60 µg
Specification heart, ventricular tissue
Gel Running Conditions Reduced Denaturing (4~12% gradient)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 01 2008

Thank you for the details that you have provided and I'm sorry to hear that you're experiencing difficulty with ab11369. In order to help improve your results, I would like to suggest the following. For IHC, it is recommended to use this primary at a d...

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