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Synthetic peptide conjugated to KLH, corresponding to amino acids 362-381 of Connexin 43/ GJA1
Our Abpromise guarantee covers the use of ab11369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent dilution.|
|ELISA||Use at an assay dependent dilution.|
|WB||1/8000 - 1/16000. Detects a band of approximately 43 kDa. Additional weak bands may be seen.|
|IHC-P||Use at an assay dependent dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
|ICC||Use at an assay dependent dilution.|
Immunocytochemistry/Immunofluorescence analysis of normoxic (A) and hypoxic (B) human mesenchymal stem cells labelling Connexin 43 / GJA1 with ab11369. Blue = DAPI (scale bars = 50 μm). Cells were grown on glass coverslips in 12-well tissue-culture dishes. Coverslips were washed with DPBS and fixed with 4% paraformaldehyde for 15 min, blocked, and incubated with the primary antibody for 1 hour at 37°C. Cells were washed and incubated with FITC-conjugated secondary antibody for 1 hour at 37°C.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"