Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab63851)

Overview

  • Product nameAnti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker
    See all Connexin 43 / GJA1 primary antibodies
  • Description
    Rabbit polyclonal to Connexin 43 / GJA1 - Intercellular Junction Marker
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Nile Tilapia
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Pig, Xenopus laevis, Non Human Primates, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Connexin 43/ GJA1.

    (Peptide available as ab92658.)

  • Positive control
    • This antibody gave a positive signal in HeLa and MCF7 Whole Cell Lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab63851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesICC/IF: Use at a concentration of 5 µg/ml.
    WB: Use at an assay dependent dilution. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionOne gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.
    • Tissue specificityExpressed in the heart and fetal cochlea.
    • Involvement in diseaseDefects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type 3 and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances.
      Defects in GJA1 are the cause of autosomal recessive oculodentodigital dysplasia (ODDD autosomal recessive) [MIM:257850].
      Defects in GJA1 may be the cause of syndactyly type 3 (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected.
      Defects in GJA1 are a cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
      Defects in GJA1 are a cause of Hallermann-Streiff syndrome (HSS) [MIM:234100]. HSS is a disorder characterized by a typical skull shape (brachycephaly with frontal bossing), hypotrichosis, microphthalmia, cataracts, beaked nose, micrognathia, skin atrophy, dental anomalies and proportionate short stature. Mental retardation is present in a minority of cases.
    • Sequence similaritiesBelongs to the connexin family. Alpha-type (group II) subfamily.
    • Cellular localizationCell membrane. Cell junction > gap junction.
    • Information by UniProt
    • Database links
    • Alternative names
      • Connexin 43 antibody
      • Connexin-43 antibody
      • Cx 43 antibody
      • Cx43 antibody
      • CXA1_HUMAN antibody
      • DFNB38 antibody
      • Gap junction 43 kDa heart protein antibody
      • Gap junction alpha-1 protein antibody
      • Gap junction protein alpha 1 43kDa (connexin 43) antibody
      • Gap junction protein alpha 1 43kDa antibody
      • Gap junction protein alpha like antibody
      • GJA 1 antibody
      • Gja1 antibody
      • GJAL antibody
      • ODD antibody
      • ODDD antibody
      • ODOD antibody
      • SDTY3 antibody
      see all

    Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker images

    • All lanes : Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab63851) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
      Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
      Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 43 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 30 kDa,55 kDa. We are unsure as to the identity of these extra bands.
    • ICC/IF image of ab63851 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63851, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, and HepG2 cells at 5µg/ml.

    References for Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab63851)

    ab63851 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (MCF7 cells)
    Specification MCF7 cells
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 07 2015

    Application Western blot
    Loading amount 100 µg
    Gel Running Conditions Non-reduced Denaturing (Upper-5% Lower 15%)
    Sample Nile tilapia (Oreochromis niloticus) Tissue lysate - whole (pituitary and heart)
    Specification pituitary and heart
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 24 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample Nile tilapia (Oreochromis niloticus) Cell (Pituitary)
    Specification Pituitary
    Permeabilization Yes - triton-X 0.3%
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 24 2014

    Application Western blot
    Sample Mouse Tissue lysate - whole (Heart)
    Loading amount 440 µg
    Specification Heart
    Gel Running Conditions Non-reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Ms. Branka Vulesevic

    Verified customer

    Submitted May 30 2012

    Merci de votre intérêt pour les anti-connexine 43 d'Abcam.

    Comme expliqué par téléphone,à notre connaissanceles anticorps http://www.abcam.com/ab62689 et http://www.abcam.com/ab63851 n’ont pas en...

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    Thank you for your inquiry.

    Unfortunately, the immunogen of ab1671 shows less than 60% homology with dog and therefore we do not expect a crpss-raction of this antibody. ab63851 does show a 93% homology with dog and would in general be eligab...

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