Anti-Connexin 45 / GJA7 antibody [5B9.2] (ab78408)

Overview

  • Product nameAnti-Connexin 45 / GJA7 antibody [5B9.2]
    See all Connexin 45 / GJA7 primary antibodies
  • Description
    Mouse monoclonal [5B9.2] to Connexin 45 / GJA7
  • Tested applicationsSuitable for: Flow Cyt, WB, ICCmore details
  • Species reactivity
    Reacts with: Rat, Rabbit, Human
    Predicted to work with: Mouse, Cow, Dog, Pig, Chinese Hamster
  • Immunogen

    Synthetic peptide:

    QAYSHQNNPHGPRE

    conjugated to KLH, corresponding to amino acids 354-367 of Human Connexin 45/ GJA7

  • EpitopeNear C terminal, cytoplasmic.
  • Positive control
    • Connexin 45 / GJA7 transfected rat C6 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab78408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ICC Use at an assay dependent concentration.

Target

Anti-Connexin 45 / GJA7 antibody [5B9.2] images

  • ab78408 staining Connexin 45 / GJA7 transfected rat C6 cells by Immunocytochemistry.
  • Overlay histogram showing HeLa cells stained with ab78408 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78408, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Connexin 45 / GJA7 antibody [5B9.2] (ab78408)

ab78408 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (muscle)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 30 µg
Specification muscle
Blocking step Blocking buffer for fluorescent western blocking as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
Username

Miss. Nathalie Da Silva

Verified customer

Submitted Jul 13 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"