Overview

  • Product name
    Anti-Connexin 45 / GJA7 antibody [5B9.2]
    See all Connexin 45 / GJA7 primary antibodies
  • Description
    Mouse monoclonal [5B9.2] to Connexin 45 / GJA7
  • Tested applications
    Suitable for: Flow Cyt, WB, ICCmore details
  • Species reactivity
    Reacts with: Rat, Rabbit, Human
    Predicted to work with: Mouse, Cow, Dog, Pig, Chinese hamster
  • Immunogen

    Synthetic peptide:

    QAYSHQNNPHGPRE

    conjugated to KLH, corresponding to amino acids 354-367 of Human Connexin 45/ GJA7

  • Epitope
    Near C terminal, cytoplasmic.
  • Positive control
    • Connexin 45 / GJA7 transfected rat C6 cells.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

Applications

Our Abpromise guarantee covers the use of ab78408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ICC Use at an assay dependent concentration.

Target

Images

  • ab78408 staining Connexin 45 / GJA7 transfected rat C6 cells by Immunocytochemistry.
  • Overlay histogram showing HeLa cells stained with ab78408 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78408, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (muscle)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
30 µg
Specification
muscle
Blocking step
Blocking buffer for fluorescent western blocking as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
Username

Miss. Nathalie Da Silva

Verified customer

Submitted Jul 13 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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